长沙烟区烟草赤星病病害调查与病原菌鉴定
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中图分类号：S435.72 文献标识码：A文章编号：1007-5119（2025）02-0045-07
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(1.湖南农业大学植物保护学院，长沙，410128；2.湖南省烟草公司长沙市公司，长沙，410023；3.湖南省长沙市农业农村局，长沙，41000；4.湖南省微生物研究院应用真菌室，长沙410009）
Investigation of Tobacco Brown Spot Disease and Pathogen Identification in Changsha Tobacco-Growing Region
XIE Lin1, CAI Hailin², LI Jianyong², ZHAI Zhengguang², ZOU Shuo³,HE Haoxin1,FU Jing1, HUANG Binbin4,LIU Qingshu4,CHEN （20
(1.College ofPlantProtection,HunanAgriculturalUniversityChangsha4028,China;2.ChangshaBranchofHunanobacco
Company,Changsha,410o3,China;3.ChangshaMunicipalBureauofAgricultureandRuralAairsofHunanProvince,Cangsha 410000,China; 4.Aplied MycologyLaboratory,Hunan Provincial Instituteof Microbiology,Changsha 4109,China)
Abstract: Brown spot disease is one of the primary factors afecting safety of leaf tobaccoquality in Changsha tobacco-growing region.Toinvestigate theocurenceandidentifythepathogenicspeciescausing tobacobrownspotdisease,acomprehensivesurvey wasconductedonincidencerateand diseaseindexofbrownspot diseaseacrossthe tobacco production towns in Changsha.Results showed that the incidence rate and disease index ranged 0 . 5 6 % - 1 6 . 6 7 % and 0.06-5.43 respectively. Through combined morphological andmolecularbiologicalidentificationofsolatedpathogens,thestudyrevealedthatthecausativeagentsereAlternariaalteata and A. tenuissima, with the detection rate of A .tenuissima up to 7 5 . 0 0 % . Further in this study, rDNA-ITS gene was confirmed to be excesive conservationforspecies-leveldiferentiation withintheAlternariagenus,whereastheplasma membraneATPasegene displayedconcordance withmorphologicalidentificationresults,provingsuitableforinterspeciesdiscriminationInconclusion,the pathogensoftobaccobrownspotdiseaseinChangshatobacco-growingregionexhibithighdiversity,withAlternariaalernatabeing predominant.
Keywords: Changshatobaco-growingregion; tobaccobrowspotdisease;pathogenidentification;A.alternata,A.tessima
烟草赤星病(tobaccobrownspot)主要发生在烟叶打顶后的成熟阶段，具有潜伏期短、暴发快等特点，适宜条件下短时间内可造成大面积流行[1]。（剩余14261字）