烟草健康株与根结线虫发病株根际微生态差异分析

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中图分类号：S435.72文献标识码：A

文章编号：1007-5119（2025）02-0026-09

Comparative Analysis of Rhizosphere Microecology Between Healthy and Root-Knot Nematode-Infected Tobacco Plants

YUAN Guoyil， WU Haol，LI Enxing²， WANG ，WANG ， ZHOU Peng1，DU ， ZHU Hongqiang3， ZHOU Yanbin³，DAI Huijuan3*， BAI Yuxiang

（1.Yunan AgriculturalUniversityKunming 6501，China;2.China TobaccoHebei IndustrialCo.，Ltd.，hijiazuang0051， China; 3. Yimen Branch of Yuxi Tobacco Company， Yuxi 653199， Yunnan， China）

Abstract： Inorder toanalyzetherelationshipbetwenrhzosphere soilmicroecologicalfactorsandtheoccurrenceoftobaccorootknotnematodedisease，atypicaltobaccofieldwithtobaccoroot-knotnematodediseasewasselectedinAziingTownship，Songing County，KunmingCity，Yunnan Province.Rizospheresoilsamples werecolected fromboth diseasedandhealthytobaccoplants. The numberofsecond-istarlarvaeofot-knotnematodes，soilchemicalproperties，enzymeactivities，andmicrobalcommunity structure were analyzed andcompared.Results showed thattherot-knot nematode pathogen was identified as Meloidogyne incognita.Comparedwiththerhzospheresoilofhealthytobaccoplants，thenumberofroot-knotnematodesintherhizospheresoilof diseased tobacco plants increasedby4.29times （ p<0 . 0 1 ） .Soil acid phosphatase activity，pH，and available phosphoruscontent were reduced by 8 6 . 9 8 % （ p<0 . 0 5 ） ， 0.33 units （ p<0 . 0 1 ） 4 5 . 5 3 % （ p<0 . 0 5 ） ，respectively. In contrast， sucrase activity increased by 5 7 . 3 6 % （204 （ p<0 . 0 5 ） .The microbialbalance，bacterialdiversityandrichness decreased，andthefungaldiversityincreasedbuttherichess decreased.ThebacterialgenusSphingomonaswereenrichedintherizospheresoilofthediseasedtobaccoplants，andthefungal phylumAscomycota wereenriched intherhizosphereofthehealthytobacoplants.Thenumberof2ndinstarlarvaeofroot-knot nematodewasignicantlypositivelyoeatedwithucraseactivityndSpngomonasndigncantlyegatielyeadih pH，acid phosphatase，availablephosphorusandAscomycotaLowsoilpHacid phosphataseactivityandavailablephosphorus content，and high sucrase activity may be important factors in inducing tobacco root-knot nematode disease.

Keywords： tobacco;rhizosphere; root-knotnematodes; microbialcommunity;soilenzymeactivity;soilchemical properties

烟草根结线虫病是一种典型的土传病害，病原根结线虫（Meloidogynespp.）在烟草不同生育期侵染烟株根系，目前防治手段有限[I。（剩余18939字）