丛枝菌根真菌种类和接种方式对烤烟幼苗生长的影响

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中图分类号：S572.01文献标识码：A

文章编号：1007-5119（2025）02-0009-09

Effect of Arbuscular Mycorrhizal Fungi Species and Inoculation Methods on Growth of Flue-Cured Tobacco Seedlings

ZHANG Yuyin1， YANG Jie1， GE Zixuan1， LI Yingying1， GUAN Kunhao1， ZHANG Tongjin²， ZHANG Yujie1， DAI Xiaoyan1* （1.CollegeofTobacco Science，Henan Agricultural University/KeyLaboratoryforTobacco CultivationinTobacondustry， Zhengzhou 450，China; 2.Bijie Branch of Guizhou Provincial Tobacco Company，Bijie 551700，Guizhou， China）

Abstract：Thisstudyaimstoclarifytheroleofarbuscular mycorhizalfungi（AMF）inthegrowthandphysiologicalcharacteristics offlue-cured tobaccoseedlings.Apotexperiment wasonductedtodetecttheimpacts of treediferentinoculationmethods andfive AMFstrains（Acaulosporascrobiculata，As；Rhizophagusintraradices，Ri；Fumeliformismosseae，Fm；Claroideoglomus etunicatum，Ce; Glomusvesiforme，Gvonthegowth prameters，potosythticpeformance，cabonandnitrogenmetabolis，and mineralnutrientuptakeoftheflue-curedtobacoVarietyK326seedlings.ResultsshowedthatthefiveAMFstrainsestablished symbiotic relationships withthe tobaccoroots under thethreeinoculation methods.Compared tothenon-inoculatedcontrol，AMF inoculationsignificantlyicreasedtheootprojectionarea，totalrotlength，oottipumber，drymaterccumulationndnet photosyntheticrateoftobaccoplants.In particular，underthe inoculation methodthree（where theinoculumwas placedapproximately away from the tobacco roots），the tobaco seedlings inoculated with Acaulospora scrobiculata （As）exhibited significantly highertotalootlength，otprojetionarea，dryateraccumulation，andetphotosytheticatethantoseinocuatedwithother AMFstrains.Furthermore，underthemethodthree，thesedlingsinoculatedwithAsdisplayedsignificantlyhigherlevelofroot diameter，drymateracumulation，netphotosyntheticrate，sucrose phosphatesynthaseactivityandnitrogencontent inthe underground parts，compared tothose inoculatedunderthe methodtwo（wheretheinoculum was indirectcontact withtheroots）. These findingssuggesthat AMFinoculation can efectivelypromote theotandleaf developmentofflue-cured tobaccoseedlings， andenhanceitsnutrientuptake.TheAsstrain，wheninoculatedunderthemethodthree，forms themost beneficialsymbiotic relationship， yielding superior results.

Keywors：arbuscularmycorzalfungi;fluecuredtobaco;ioculationmethod;growth;physiologicalcharacteristics;ieral nutrients

烤烟壮苗培育是优质烟叶生产的基础，直接影响烟株大田生长发育及烟叶产量和品质[1]。（剩余17101字）