柱前衍生HPLC法分析烟叶水溶性多糖的单糖组成

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，(1.，厦门；2.，厦门361005）

中图分类号： 文献标识码：A

文章编号：1007-5119（2025）02-0052-08

Analysis of Monosaccharide Composition of Tobacco Water-soluble Polysaccharides by Pre-column Derivatization HPLC

ZHAO Yan12,HUANG Yanjun1,LIU Xiucai1, HUANG Huafa1, LIU Jiangsheng1, YE Zhonglil, ZHANG Tingguil,LIU Zechun1, XIE Wei1*,LI Yaoqun2* (1.TechnologyCenterofChinaTobaccoFujianIndustrialCo.,Ltd.,Xiamen36101,China;2.ColegeofChemistryandCheical Engineering, Xiamen University, Xiamen 3610o5, China)

Abstract:Adeterminationmethod was established forthe monosaccharide compositionandcontentof tobaco water-soluble polysacharides by1-phenyl-3-methyl-5-imdazolinone(PMP)pre-columnderivatizationhigh performance liquidchromatography. Completeacidhydrolysis method was employedtohydrolyze tobacco water-solublepolysaccharides,andthe hydrolysis products weresubjectedtoPMPderivatizationreaction.Thesignalofmonosaccharidederivativesof tobacowater-solublepolysaccharides afteracid hydrolysis was determinedbyreversephase high-performance liquidchromatography.Themethod wasusedto explorethe monosaccharidecompositionandcontentof tobaccowater-solublepolysaccharidesofdiferentregionsandtypes.Theoptimized detection conditions were as follows: 0 . 0 5 m o l / L phosphate( -NaOH,pH7.O) buffer (solvent A) and acetonitrile (solvent B) were chosen as the mobile phase of elution system at the isocratic elution condition ( 8 4 : 1 6 ) ,with Agilent Eclipse XDB-C18 column ( 2 5 0 m m×4 . 6 m m, ; theUV detection wavelength was 2 5 0 n m .Results showed that the linear relationship is good for the nine monosaccharides in the concentration range of 2 0 . 0-8 0 0 μ g / m L ，with the correlation coefficient .The limits of detection and quantification were 0.07-0.17 and 0 . 2 2 -0 . 5 6 μ g / m L ，respectively. The average recoveriesranged 8 2 . 3 % - 1 0 4 . 0 % in spiked samples.Thetobacowater-solubleposaccharideswerecomposedofmanose (Man)hamose(Rha),galactose(Gal)aboe (Ara),glucose(Glc)，glucuronicacid (GlcUA)，and galacturonicacid (GalUA)，with Glcand GalUA acounting forahigher proportion.There were significant differences inthe monosaccharidecompositionof tobaco water-soluble polysaccharides from variousregionsandtypes.Themethodisfast,simple,highlysensitive，andhasgoodrepeatability，thuscanbeused for monosaccharidecompositionanalysisof tobaccopolysaccarides.At thesametime,itprovidesabasisforfurtherresearchonthe structure of tobacco polysaccharides.

Keywords: tobacowater-solublepolysaccharides;pre-coumnderivatizatio;iquidchromatography;monosaccaridecoositon

多糖是不同类型的单糖残基通过多种类型的糖昔键连接而成的天然聚合物，与卷烟感官品质息息相关。（剩余24041字）