烟草靶斑病拮抗菌Y18鉴定及发酵条件优化

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中图分类号：S435.72 文献标识码：A文章编号：1007-5119（2025）02-0035-10

¹，熊有明²，²，刘峰³，母婷婷²，彭静4，杨未²， 1，¹，5*，1*

1.，；2.，；3.，；4.，；5.，）

Identification of Tobacco Target Spot Antagonistic Strain Y18 and Optimisation of Its Fermentation Conditions

XIAO Yuel, XIONG Youming², XIAO Zhipeng², LIU Feng³, MU Tingting², PENG Jing4, YANG Wei²,LI Lingling1,MEI Siyi1,LIU Tianbo5*,TANG Qianjun1*

(1.CollgeofPlantProtectio,HunanAgriculturalUniversity,Changsha428,China;2.HengyangBranchofHunanoba

Company,Hengyang 41oo1,Hunan,China;3.ongzhouBranchofHunan TobaccoCompany,Yongzhou450o,Hunan,Cina; 4.HunanPlantProtectionInstitute,Changsha41l25,China;5.HunanTobaccoResearch Institute,Changsha414,China)

Abstract:Tobacoargetspotdisease,causedbyizoctoniasolaniisoneofthemostimportantfoliariseasesoftobaccoe screeningandidentificationofbocontrolstrains,along withtheestablshmentofbocontrolfermentationconditions,cantecicaly supprtthepreventionandcontrolof thedisease,andtheaplcationofbiocontrolbacteria.Inthisstudya previouslysreeed antagoniststrainY18 wasidentifiedthroughthecombiationofmorphology,physiology,biochemistryandmolecularbiology.Using theinhibitionrateofY18onthemycelialgrowthoftargettobaccoblotchasanindex,thefermentationmediumcomponentsand fermentationconditions withtheoptimalinibitoryefectofY18onthetarget bloth were screened byone-waytest andorthogonal test.The strain Y18 was identified as Bacillusvelezensis,with the inhibitionrate of 8 1 . 6 3 % on Rhizoctonia solani. It also had pronouncedinibitiononPhytophthoracapsici，Fusariumgraminearum，Phytophthoraparasiticavar.nicotiane,Ralstona solanacearum and Pseudomonas syringaepv. tabaci,respectively,with the inhibitory rate reaching 7 9 . 0 8 % ,and the size of the inhibition circle for Ralstonia solanacearum up to 2 7 . 2 8 m m .The optimal fermentation medium, carbon source, nitrogen source and inorganicsalt wereNBmedium,sucrose,beef pasteandmagnesiumsulphate,respectively.Theoptimalmediumcomponents were sucrose, beef paste and magnesiumsulphate.The optimal fermentation conditions were p H=6 ，fermentation timeof ,inoculum amount of 5 % and fermentation temperature of . The resultscan provide a theoretical basis for the development and utilization of strain Y18 as a biocontrol agent.

Keywords: tobacco target spot; Bacillus velezensis; antagonistic effect; fermentation optimization

烟草靶斑病是由立枯丝核菌(Rhizoctoniasolani)引起的真菌病害[1]，在我国主要烟区均有发生[2-3]，给烤烟生产带来严重经济损失。（剩余31322字）