基于特征指纹图谱的金线风生物碱提取物质量评价

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【中图分类号】R284.1 【文献标志码】A 【文章编号】1007－8517（2025）11-0055-05

DOI： 10.3969/j .issn.1007-8517.2025.11. zgmzmjyzz202511012

QualityEvaluationofAlkaloidExtracts ofCyclea Hypoglauca Basedon CharacteristicFingerprint

ZHANG Yunyun1²YANG Haichuan1.2*ZHOU Qi3 LAI Bingxia³ 1.Guangxi Institution of chinese Medicine Pharmaceutical Science，Nanning 53OO22，China; 2.Key Laboratory forResearchon Quality Standards of Traditional Chinese Medicinein Guangxi，Nanning 530022，China; 3.YoujiangMedicalUniversityForNationalities，Baise533OOO，China

Abstract：ObjectiveToestablishthecharacteristicfingerprintofalkaloidextractofCyclea hypoglauca，anddevelopHPLC method for determination of ⊥- curarine content. Method The chromatography was performed on Agilent ZORBAX XDB -C （4.6 18 mm×75mm 3.5μm） column，Acetonitrile（A） -0.05mol/L potassium dihydrogen phosphate solution containing 0.2% triethylamine （ pH=3 ）（B）waselutedbymobilephasegradient， λ=210 nmwasused toanalyzeand determine.ResultsHPLC fingerprint of alkaloidextractofCycleahypoglaucawasestablished，2Ocommonpeakswereidentified，and11batches werehighlysiilar.L curarine showed a good linear relationship in a certain concentration range，with R=0 .9998.The precision，repeatability，stability and RSD of sample addition recovery tests were all less than 3.0% ，and the average recovery rate of sample addition recovery was 98.11% .Conclusion Themethod hashigh precision，goodrepeatabilityand stability，and theresultsareacurateand reliable， which is suitable for the quality control and evaluation of alkaloid extract of Cyclea hypoglauca .

Key words： AlkaloidExtracts of Cyclea hypoglauca;Fingerprints；L -curarine；HPLC

金线风为防己科轮环藤属（CycleaAm.exWight）植物粉叶轮环藤的Cycleahypoglauca（Schauer）Diels的干燥根。（剩余5282字）