HPLC法测定参芪通络颗粒中羟基红花黄色素A的含量

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【中图分类号】R284.1 【文献标志码】A 【文章编号】1007-8517（2025）11-0052-03

DOI： 10.3969/j . issn.1007-8517.2025.11. zgmzmjyyzz202511011

Determination of Hydroxysafflower Yellow A in Shenqi Tongluo Granules by HPLC

LIN Qunfeng'XU Jie²*ZHANG Huijing² LI Ning²LI Qiang² 1. ， ， ，; 2. ， ，

Abstract：ObjectiveToestablishahighpeformance liquidchromatographymethodforthequantificationof hydroxysafflower Yellow A in Shenqi Tongluo granules. Methods After dissolved with 50% methanol，ultrasonic， filtered，the test sample was loaded onto a YMC - Triart C18 （ 250mm×4.6mm ， 5μm ）column to separate with a mobile phase of methanol -acetonitrile -0.7% （2 phosphoric acid（30：2：68），the detection wavelength was 403nm ，the column temperature was 30 C ，the injection volume was 10 （20 μL ， the flow rate was 1.0mL/min . Results The calibration curve of hydroxysafflower yellow A showed a good linearity（ R2=1 ） in the range of32.55-651 ng.The recoverieswere in the range of100.17%-102.64 % ， the relative stard deviations（RSD） was 0.87% .Conclusion The method issimple，accurate，reproduciblestable，can beused forthedeterminationof hydroxysafflower yellow A in Shenqi Tongluo granules.

KeyWords：Shenqi Tongluo Granules；Hydroxysafflower Yellow A；Content Determination；HPLC

参芪通络颗粒处方来源于寿光市中医医院临床经验方。（剩余4235字）