HPLC法同时测定黄连胶囊中4种成分的含量

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【中图分类号】R284.1 【文献标志码】A 【文章编号】1007-8517（2025）11-0048-04

DOI： 10.3969/j . issn.1007-8517.2025.11. zgmzmjyyzz202511010

Simultaneous Determination of Four Componentsin Huanglian Capsules by HPLC

ZHANG Huowang' ZHANG Dongxia1* LAI Lihuan² 1.Heyuan Institute for Drug Control，Heyuan 517OOO，China; 2.Medical CollegeofJiayingUniversity，Meizhou 514OoO，China

Abstract：Objective To establishan HPLC method for simultaneous determinationofberberine hydrochloride，epiberberine， coptisine hydrochloride and palmatine hydrochloride in Huanglian Capsules.Methods CAPCELL PAK C18 chromatographic column （ 250mm×4.6mm ， 5μm ）was used. The mobile phase was acetonitrile -0.05mol/L potassium dihydrogen phosphate solution （45：55），isocratic elution，flow rate 1.0mL/min ，detection wavelength 345nm ，column temperature 30qC ，injection volume：10 （20 μL .ResultsBerberinehydrochloride，epiberberine hydrohloride，coptisinehydrochlorideandpalmatinehydrochloridehadgoodlinear relationship with the peak area in the linear ranges of 3.9041-23.4480μg/mL ，0.3644 -2.1888 μg/mL， 0.6711-4.0304μg/ mLand 0.8502-5.1064μg/mL ，respectively. The average recoveries were 99.61% 98.39% ，98.84 % ， 99.04% ，and the RSDs were 0.19 % ，1.21 % ，0.78 % ， 0.53% （ n=6 ），respectively. Conclusion The method is efficient，accurate and sensitive， andcanbeusedforthesimultaneousdetermiationofberberinehydrochloride，epiberberine，coptisinehydrochlorideandpalmatine hydrochloride inHuanglian Capsules，which providesascientific basis forbetercontrolofthequalityofHuanglian Capsules.

Keywords：Huanglian Capsules；HPLC；ContentDetermination

黄连胶囊是由黄连制成的单方制剂，具有清热燥湿、泻火解毒的功效，用于湿热蕴毒所致的痢疾、黄疸，症见发热、黄疸、吐泻、尿黄如茶、牙龈肿痛或大便脓血。（剩余4388字）