基于BSA-seq技术定位调控西瓜红色果肉形成基因

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中图分类号：S642.9 文献标识码：A 文章编号： 1000-4440（2025）05-0952-08

Abstract：Flesh colorisanimportant trait thatdetermines thequalityof watermelon fruit.Inthisstudy，weused the pink-fleshed watermelon MW119 and the red-fleshed watermelon MW120 as parents to construct an F2 segregating population. Fromthis population，weselected individuals withredflesh and pink flesh phenotypes tocreate progenybulks.Wethen performed whole-genome resequencing with a coverage depth of 30 1× on both the parental and progeny bulks using BSA-seq technology.By using the Δ （SNP-index）method，we identified 12 intervals significantlyassociated with the red flesh trait on chromosomes2，4，5，6，7，9，nd1，withatotallengthof56.66Mb.Furtherfunctionalaotationandexpresionaalyis wereconductedon116genes withinthesignificantlyassociated intervalsthatcariednon-synonymousSNPandInDelframeshift mutations，ultimatelyleading totheselectionoffivekeycandidategenes：the WRKY transcriptionfactorgene Cla97C02G028430，the o -methyltransferase gene Cla97C02G028680，the F-box protein genes Cla97C02G048400 and Cla97C06Gl22530，and the pentatricopeptide repeat protein gene Cla97Co6G122120.These genes playa significant role in regulating the formation ofred fleshinwatermelon.The resultsof thisstudy furtherrefine themolecular mechanisms underlyingtheformation ofredfleshinwatermelonand providea theoretical basis for marker-assisted breeding.

Key words： watermelon；flesh color； gene map-ping

西瓜（Citrulluslanatus）是重要的园艺作物，因其色泽鲜艳，果肉甘甜多汁而深受消费者喜爱。（剩余13378字）