基于转录组解析玉米苗期根系对非生物胁迫的响应

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中图分类号:T513 文献标识码:A 文章编号:1000-4440(2025)05-0848-10
Abstract:Abioticstreses(drought,salt)havebecomeoneof thekeyfactorsrestrictingtheyieldandqualityof maize. To elucidatethemechanismsof maizeresponsedtoabioticstresses,inthisstudy,B73wasusedastheexperimentalmaterial, and four treatments were set up: control (CK),drought stress treatment (D),salt stresstreatment (S)and drought + salt compoundstresstreatment(DS).Primaryroot length wasdynamicallymonitored,andtheroot transcriptome wassequenced.Theresultsofphenotypicanalysisshowedthattheprimaryrotlengthwassignificantlyshorterunderdiferenttreatmentscomparedwith the control. A total of 1 526 differentially expressed genes were identified in the comparison groups between the control and diferentstresstreatments.Atotalof2O7,17Oand1274diferentiallexpresedgeneswereidentifedinthecomparisongroupsbetween the control and drought stress,salt stress and drought + salt compound stress. Functional enrichment results suggested that thesediferentiallyexpressed genesweremainlyinvolvedinthebiologicalproceses mediatedbyabscisicacidsynthesisandsignal transduction,and transcription factors in response to abiotic stress.MYB and ERF family transcription factor genes showed the
greatest response to abiotic stress.The results of this study layafoundation for further revealingthemolecular mechanism of maize seedlings in response to abiotic stress.
Key words:maize;abiotic stress;transcriptomics;differentially expressed genes;abscisic acid
玉米(ZeamaysL.)是世界上重要的粮食、饲料和经济作物,在农业生产和经济发展中占据重要地位[]。(剩余15708字)