马铃薯无糖组织培养的条件优化及其在马铃薯原原种生产中的应用

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Abstract：Tofurtheroptimizethemicroenvironmentparametersofsugar-free tisseculture，this studyused Qingshu No.9 as the tst material and designed different gradient experiments with diferentratiosof vermiculite to nutrient solution （ Vvermiculite ： 1 Vnutrientsolution=1.0：0.7，Vvemiculie：Vnutrientsolution=1.0：0.8 mtriensoution =1.0：0.9），COconcentrtions（500 μmol/mol， 800μmol/mol ， 1100μmol/mol ， 1 400μmol/mol ），and light intensities to investigate the effectsof each treatment

onthe growth and development of potato tissue-cultured seedlings.Thelight intensitywasset at 50μmol/（m2⋅s） 75μmol/（m2⋅s） ， 100μmol/（μ2⋅s）. The results showed that the optimal conditions for sugar-free tissue culture of potatoes were as follows ： ， CO2 concentration of 800μmol/mol ，and light intensityof75 μmol/（m2⋅s）. Compared with traditional tissue culture，the yield，quantity，and survival rate of potato pre-basic seeds obtained through sugar-free tissue culture were significantly increased by 16.09% ， 22.37% ，and 3.27% ，respectively（ P<

0.O5）.Inconclusion，thisstudynotonlyclarifidthekeytechnicalparametersofsugar-freetissueculturebutalsoonfimedthe advantagesoftistechologyinimprovingteproductionefiencyofpotatoseedtubers，providingteoreticalbasisadtecal support for the standardized production and industrial propagationof potato tissue-cultured seedlings.

Key words：sugar-free tissue culture；potato；tissue-cultured seedlings

植物无糖组织培养技术由日本千叶大学古在丰树教授发明[1]，其技术核心在于通过精确控制微环境因素（如 CO2 浓度、温度、湿度等），以环境 CO2 替代培养基中添加的外源糖作为碳源，实现植物完全自养生长。（剩余10515字）