基于成年优树埋干复幼的小黑杨组培体系建立

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中图分类号：S792.11;S723.1 文献标识码：A doi：10.13601/j.issn.1005—5215.2025.06.011

Establishment of Tissue Culture System for Populus simonii ×P . nigra BasedonStemBurial Rejuvenation of Adult Elite Trees

Han Ruilong1，Ji Xiaohui²，Si Si²，Shi Shaolin1 * （1.College of Life Science and Agroforestry，Qiqihar University，Qiqihar l6looo，China; 2.Qiqihar Branch of Heilongjiang Academy of Forestry，Qiqihar 16loo5，China）

Abstract To establish a tissue culture system for elite trees of Populus simonii ×P . nigra via stem burial rejuvenation，semi-lignified branches from cuttings of rejuvenated adult elite trees of Populus simoni ×P . nigra after stem burial treatment were used as explants.The optimalexplant disinfection method was screened，and conditions suitable for callus induction from Populus simonii ×P . nigra leaves were explored，aiming to establish an efficient and stable tissue culture system for Populus simonii ×P . nigra .The results showed as the following four aspects. （1）The combination of 75% alcohol disinfection for l2O seconds and 0.1% （20 HgCl2 disinfection for7 minutes had the best effect，with a contamination rate of 17.33% .（2）The optimal medium formula for leaf callus induction of Poϕ 1 ulus simonii ×P . nigra was M 1S+0.05mg ⋅L-1NAA+0.5mg L-16-BA+30g ⋅L-1 sucrose，under which the induction rate was 70% .（3） In the proliferation stage，the optimal medium formula was MS+O.O5 mg L-1NAA+30 （204号 g L-1 sucrose，with a proliferation coefficient of 8.32.（4） The optimal seedling strengthening medium for tissue culture seedlings of Populus simonii ×P . nigra was 1/2MS+0.01mg L-1NAA+0.05mg L-1IBA+20g L-1 sucrose，and the growth amount under this culture condition reached 9.37cm

Key wordsPopulus simonii ×P . nigra; tissue culture;semi-lignified branches;explant disinfection; screening of culture medium

农田防护林具有减少土壤风蚀、防风固沙、保护农田生态系统，调节周围大气、植物和土壤环境，维持农田小气候平衡等重要功能，是农田生态系统的屏障，对改善人类环境质量具有重要意义[]。（剩余7462字）