小叶杨DREBID基因的克隆及单核苷酸多态性（SNP）分析

打开文本图片集

中图分类号：S722.3 文献标识码：A doi：10.13601/j.issn.1005-5215.2025.05.003

CloningandSNPAnalysisoftheDREBlDGenein Populussimonii

Wang Jianyi

（ShanxiAcademyofForestryand Grassland Sciences，Taiyuan O3oo12，China）

AbstractInordertofullyexploretheexcelentstressresistanceofPopulussimonii，teDREBIDgeneofPopulussimoniiwasloned andsequenced，anditsgenesequenceandlinkagedisequlibriumcharacteristicswereanalyzed.TheresultssowedthattheEB geneof Populussimoni hadnointron，andthelengthof DREBIDgene was8Oo bp.Thegenecontainedacompleteopenreading frameand could encode a protein witha length of240amino acidresidues.The molecular weightof the protein was about ， andthe isoelectricpoint was4.85.The homologyofDREBIDsequence betweenPopulussimoni andPopulus trichocarpa wasashigh as 90% .TheSNP frequencyinthegenewas0.04375，andthere were5commonsingle nucleotidepolymorphisms（SNPs），30rare SNPs，24misensemutationsand11nonsensemutations.ThelinkagedisequilibriumhasbeendegradedintheDREBIDgeneof Populus simoni.

eyWordsPopulus simonii;DREBID gene;transcription factor;SNP;linkage disequilibrium

小叶杨（Populussimonii）是我国北方重要的乡土树种，以其抗旱、耐低温和耐瘠薄等特性，在生态建设和经济发展中占据重要地位。（剩余8960字）