化橘红均一多糖的制备及其抑制HepG2细胞增殖的研究

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关键词：化橘红；均一多糖；水提醇沉法；HepG2 细胞；细胞增殖；药效活性中图分类号：R284.2文献标志码：A 文章编号：1003-9783（2025）07-1157-06doi：10.19378/j.issn.1003-9783.2025.07.014

Abstract：Objective To isolate and purify homogeneous polysaccharides from Citri Grandis Exocarpium （CGEHP） and investigate their effects on HepG2cell proliferation.Methods Crude polysaccharides were extracted via water extraction and alcohol precipitation，followed bypurification using DEAE-52 cellulose and Sephacryl S-400 HR chromatography columns to obtain CGEHP.The homogeneity of CGEHP was verified by UPLC-SEC-ELSD，while monosaccharide composition was analyzed by GC-MS.The inhibitory effcts of CGEHPon HepG2 cellviability were examined.Results Two homogeneous polysaccharides（CGEHP-1 and CGEHP-2）were isolated.CGEHP-1 exhibited a molecular weight of 11 310.56 Daand was composed of mannose，arabinose，rhamnose，and glucose，whereas CGEHP-2 showed a molecular weight of 8112.34 Da and was composed solelyof mannose，demonstrating higher homogeneity.Significant inhibitionof HepG2cellproliferation was observedat CGEHP-1and CGEHP-2 administration concentrations of 400μg⋅mL-1 and 50μg⋅mL-1 ，respectively. Conclusion The established method for CGEHP preparation is reliable.Both CGEHP-1 and CGEHP-2 exhibit inhibitory efectson HepG2cell proliferation，with CGEHP-2 demonstrating a more potent inhibitory activity.This study provides a foundation for further research on the pharmacodynamic material basis of Citri Grandis Exocarpium.

KeyWords：Citri Grandis Exocarpium；homogeneous polysaccharide；water extraction and alcohol precipitation; HepG2 cells；cell proliferation； pharmacological activity

化橘红是岭南道地药材，为芸香科植物化州柚Citrusgrandis‘Tomentosa’或 柚 Citrusgrandis（L.）Osbeck未成熟或近成熟的干燥外层果皮，具有理气宽中、燥湿化痰的功效，临床用于治疗咳嗽痰多、食积伤酒、呕恶痞闷等症。（剩余8470字）