不同产地大黄的结合型蒽醌含量与泻下活性的相关性研究

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关键词：大黄；结合型蒽醌；泻下活性；相关性分析；高效液相色谱法；泻下指数；炭末推进率；小鼠中图分类号：R285.5文献标志码：A文章编号：1003-9783（2025）07-1064-07doi： 10.19378/j.issn.1003-9783.2025.07.004

Abstract：Objective To investigate the corelation between the content of six conjugated anthraquinonesand the purgative ffcts of Rheum palmatum from diferent origins.Methods The contents of aloe-emodin-8- o -glucoside （A8G），rhein-8-O-glucoside（R8G），chrysophanol-8-O-glucoside（C8G），emodin-8-O-glucoside（E8G）， physcion-8- υ -glucoside（P8G），and sennoside A in Rhei Radix et Rhizoma from 1O geographical origins were determined using high-performance liquidchromatography（HPLC）.The purgative efect of Rhei Radix et Rhizoma decoction onnormal mice were evaluated based on purgative index and charcoalpropulsion rate.Grey relational analysis and corelation analysis wereemployed to explore therelationship between thecontent of six conjugatedanthraquinones andpurgative fects.Results Statisticallysignificant differences wereobserved inthecontent of sixconjugated anthraquinones among the 1O origins （ P<0.05 ）.Rhei Radix et Rhizoma from Enshi，Hubei Province，exhibited the highest levels of A8G，R8G，C8G，P8G，and sennoside A，while samples from Haiyuan，Ningxia，showed the highestE8Gcontent.Significant variations in purgative efects（purgative index and charcoal propulsion rate）were observed across origins，with Rhei Radix et Rhizoma from Enshiand Jianshui demonstrating the strongest effects （ P< 0.05）.Greyrelationaland correlationanalyses revealed apositive corelationbetweenconjugatedanthraquinones content andpurgative effects.A8G，C8G，P8G，and sennoside A contributed significantly to both purgative index and charcoal propulsionrate.Conclusion The purgativeeffects ofRhei Radix et Rhizoma arises from thesynergistic effects of multiplecomponents，with A8G，C8G，P8G，and sennoside A likelyserving as key active constituents.These findings provide a foundation for further exploration of the purgative mechanism of Rhei Radix et Rhizoma.

Keywords：Rhei Radix et Rhizoma；anthraquinone glycoside；purgative effect；corelationanalysis；high-performance liquid chromatography； purgative index； charcoal propulsion rate； mice

大黄为蓼科植物掌叶大黄（RheumpalmatumL.）、唐古特大黄（RheumtanguticumMaxim.exBalf.）或药用大黄（RheumoffcinaleBaill.）的干燥根及根茎，具有泻下攻积、逐瘀通经、清热泻火、凉血解毒、利湿退黄等功效[2-3]，是中药组方中应用范围广泛的高频中药之一[4。（剩余10219字）