基于代谢组学探讨消食白头翁汤治疗溃疡性结肠炎的疗效及机制

打开文本图片集

关键词：消食白头翁汤；溃疡性结肠炎；代谢组学；苷类代谢；嘧啶代谢；小鼠中图分类号：R285.5 文献标志码：A文章编号：1003-9783（2025）07-1123-08doi：10.19378/j.issn.1003-9783.2025.07.010

Abstract：Objective To investigate the therapeutic efects andunderlying mechanisms of Xiaoshi Baitouweng Decoction（XBD）inulcerativecolitis（UC） mice.Methods Mice were randomized into normal，model，Mesalazine， XBD low-dose，and XBD high-dose groups using a random number table.A UC mouse model with "damp-heat syndrome of the large intestine" was established via a "high-fat high-sugar diet + alcohol + artificial climate incubator" protocol combined with 3% dextran sodium sulfate （DSS）. Corresponding treatments or pure water were administered via gavage over a 22-day modeling period.Disease activity index （DAI）and histopathological scores were calculated， colon length was measured，and colon morphology was assessed via hematoxylin-eosin（HE） staining.ELISA was used to detect IL- 1β and TNF- α levels in colon tissues，while ultra-high-performance liquid chromatography coupled with Fourier transform mass spectrometry（UHPLC-FTMS）analyzed colonic metabolite profiles to identify diferential metabolites and asociated pathways.Results Compared with the normal group，the model group exhibited elevated DAI scores （ P<0.001 ）and histopathological scores （ P<0.01 ），shortened colon length （ P<0.001 ），and increased IL- 1β and TNF- α levels（ P<0.01 and P<0.001 ）.Compared with the model group，the XBD high-dose group showed reduced DAI scores（ P<0.05 ）and histopathological scores（ P<0.01 ），longer colon length（ P<0.01 ），decreased IL- 1β and TNF -α levels（ P<0.05 and P<0.01 ），and significant alleviation of clinical symptoms.Metabolomics revealed that XBD primarily modulated nucleoside-related metabolites and pathways including pyrimidine metabolism， glycerophospholipid metabolism，tyrosine metabolism，and primary bileacid biosynthesis.Conclusion XBD exerts therapeutic efectson UC，potentially mediated byregulating microbial nucleoside metabolism andinfluencing pyrimidine metabolic pathways.

Keywords：Xiaoshi Baitouweng Decoction；ulcerative colitis；metabolomics；nucleoside metabolism；pyrimidine metabolism；mice

溃疡性结肠炎（Ulcerativecolitis，UC）是一种肠道非特异性炎症性疾病，属于炎症性肠病（Inflammatoryboweldisease，IBD）的一种，其重要特征是肠道炎症和肠上皮结构破坏，该病慢性且反复迁延，是结肠癌发病的重要危险因素[-2]，因此寻找安全有效的溃疡性结肠炎治疗手段具有重要临床意义。（剩余11109字）