UPLC-MS/MS法同时测定参苓白术颗粒中12种成分的含量

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关键词：参苓白术颗粒；桔梗皂苷D；竹节参皂苷Ⅳa；拟人参皂苷 F11 ；UPLC-MS/MS；含量测定；质量评价中图分类号：R284.1文献标志码：A文章编号：1003-9783（2025）07-1151-06doi： 10.19378/j.issn.1003-9783.2025.07.013

Abstract：Objective To establish an ultra-performance liquid chromatography-tandem massspectrometry（UPLCMS/MS）method for simultaneous quantificationof 12 active components in Shenling Baizhu Granules.Methods Chromatographic separation was performed on a Waters Atlantis T3 column （ 4.6mm×75mm ， 3μm ）at 30∘C ， with a mobile phase consisting of 0.1% formic acid in water and methanol under gradient elution.The flow rate was （202 0.4mL⋅min-1 ，and an electrospray ionization （ESI） source was employed for detection in both positive and negative ion multiplereaction monitoring（MRM） modes.Results The 12 active components [platycodin D，chikusetsusaponin Na ， pseudoginsenoside F11 ，allantoin，liquiritin，atractylenolideI，atractylenolide II ，atractylenolide Il ，ginsenoside Rf，ginsenoside Rb1 ，ginsenoside Re，and ginsenoside Rgil exhibited good linearity （ r>0.9977 ）within their respective concentration ranges.The average recoveries ranged from 96.16% to 102.13% with relative standard deviations（RSD） <4% .Significant variations in the content of these 12 components were observed across eight batches of Shenling Baizhu Granules.Conclusion This method is simple，accurate，and reproducible，providing areliable approach for quality control and research of Shenling Baizhu Granules.

Keywords： Shenling Baizhu Granules； platycodin D；chikusetsusaponin Va； pseudoginsenoside F11 ； UPLC-MS/MS；content determination；conten analysis

参苓白术颗粒是基于中医理论研发的复方制剂，由人参、茯苓、麸炒白术、山药、炒白扁豆、莲子、麸炒薏苡仁、砂仁、桔梗、甘草等10味药材结合现代制药技术精制而成的制剂。（剩余7524字）