基于“三因制宜”理论探讨“湿热型”内外环境对溃疡性结肠炎与肠道菌群关系的影响

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关键词：溃疡性结肠炎；饮食环境；肠道微生物群；脂质代谢；炎症反应；湿热；小鼠中图分类号：R285.5文献标志码：A 文章编号：1003-9783（2025）07-1131-14doi：10.19378/j.issn.1003-9783.2025.07.011

Abstract：Objective To investigate the influence of "damp-heat typed"internal and external environmentson ulcerativecolitis（UC）and itsunderlying mechanisms，particularlytheregulatoryroleof gut microbiota in UC pathogenesis，guided bythe "treatmentinacordance with three categories of etiological factors" theoryand combined with theunique"damp-heattyped"dietary environment in China's Lingnan region.MethodsA dextran sulfate sodium （DSS）-induced UC mouse model was established under dietary and environmental interventions.External damp-heat conditions（high temperature and humidity）and internal damp-heat factors （high-sugar，high-fat dietwith alcohol intake）were simulated to replicate theefects ofa"damp-heat" environmenton UC progression.Biologicalsamples were collected to analyze objective indicators，including inflammatorycytokines，aquaporins，lipid profiles，and gut microbiota composition.Fecal microbiota transplantation（FMT）was employed to elucidate therole of gut microbiota in mediating the efects of the "damp-heat" dietary environment.ResultsUnder the "damp-heat typed" environments， UC miceexhibited elevated disease activity index （DAI）scores，shortened colon length，disrupted epithelial structure， increased proinflammatory cytokine levels，pathological alterations in lipid metabolism，and dysregulated aquaporin expression.16SrRNA sequencing revealedan increased abundance ofProteobacteria and decreased Firmicutes in the gut microbiota.FMT experiments confirmed thatthe "damp-heattyped" environments exacerbated UC through detrimental modulation of gut microbiota.Conclusion The "damp-heat" internal and external environments aggravate DSS-induced UC byreshaping gut microbiota，thereby disrupting lipidfluid metabolism andamplifying inflammatory responses. These findings provide a clearer understanding of the pathophysiological interplay between dietary environments and UC progression.

Keywords：ulcerative colitis；dietary environment；gut microbiota；lipid metabolism；inflammatory response; damp-heat；mice

溃疡性结肠炎（ulcerativecolitis，UC）是一种影响肠道黏膜及其下层的慢性反复发作的炎症性肠道疾病，临床以腹泻、便血、腹痛为特征-2]。（剩余22798字）