诺如病毒快速免疫层析检测效能的评估及应用

打开文本图片集

关键词：诺如病毒；免疫层析；检测效能

中图分类号：R373.2文献标志码：A 文章编号：2097-7174（2025）11-1069-04

DOI：10.3969/j.issn.2097-7174.2025.11.007

Abstract：Objective：To evaluate detection performance of anorovirus immunochromatographic（IC）test kit and provide evidence for itspractical application.Methods：Atotalof10O diarrheal samples werecollectedand tested in parallel using the IC test kitandtheRT-qPCR methodsrecommended by quarantine industrystandards.The detection effcacyoftheICtestkitfornorovirusanditsgenotyping wasaessdthroughstatisticalanalysisofresultsfromboth methods.Results：The sensitivity，specificity，and concordance rate of the IC detection kit reached 96% 100% ，and 97% ，respectively. Compared with the standard detection methods，the MeNemar chi-square value was 1.333，and the P -valuewas O.248，indicatingnostatistical diference.Theresultsoffurtherdetectionof differentnorovirusgenotypes showed that theIC detectionkits demonstrated asensitivityof 100.0% for the norovirusGI.5，GI.3，andGⅡ.7 genotypes， while the sensitivities for the G II .4 and G II.17 genotypes were 94. 4% and 87.5% ，respectively. Conclusion： TheICdetection kithashighsensitivityandspecificity，thedetectionresultsshowhighconsistencywithRT-qPCR detection，making it suitable as a screening method for norovirus in primary healthcare institutions.

Keywords：Norovirus；Immunochromatographic；Detection performance

诺如病毒（Norovirus，NoV）是全球急性胃肠炎（Acutegastroenteritis，AGE）暴发的主要病原体，约20% 的急性胃肠炎与诺如病毒感染有关[1]。（剩余4645字）