多壁碳纳米管生根培养基对草莓脱毒苗生长的影响

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Effect of Multi-walled Carbon Nanotube Rooting Medium on the Growth of Strawberry Virus-free Seedlings

CAO Fan，JINYu-qing，GU Qing-qing et al（Jiangsu Yanjiang Institute of Agricultural Sciences，Nantong，Jiangsu

AbstractObjective]Toestablishastrawberytisueculturesystembasedoncarbonnanotubemediu.[MethodThistudyusedHongyan’strawberseexpietalmaterialtoexploetheifueneofulti-alldarbnoubeoigddifntota tions （25，50 and 100mg/L ）onthe growth of strawbery tisue culture seedlings，and observed the growth of adventitious roots.[Result]The results showedtataddingulti-walledcarbonnanotubes tothestrawberryotingculturemedmsignificantlyimprovedtecalduction rate. Compared with the control group， 50mg/L multi-walled carbon nanotube rooting medium had the best efct， increasing the height of virus-free seedlings by 27.1% ，the total root length by 83.4% ，the total root volume by 163.8% ，and the number of root tips by 70.1% .Microstructuralobservatiosevaledthattheuli-walldarbnnaotubseeabsobdotesufceofthdventitiousosnddidotg glomerate inside them.The survival rate of transplanting after acclimatization was above 95% ，and the young seedlings grew well. [Conclusion] In this study，a multi-walled carbon nanotube rooting medium （ 50mg/L ）suitable for strawberry tissue culture was selected to provide technical support for the application of carbon nanomaterials in strawberry propagation.

Key Words‘ Hongyan’strawberry;Carbon nanotube;Tissue culture;Microstructure;Adventitious root

碳纳米管（carbonnanotubes，CNTs）是一种长宽比例大于1000的一维纳米级碳结构，具有独特的机械性、导热性和电学性能，可改变化学反应或生物结构[1-2]。（剩余6776字）