陕西石蒜鳞茎膨大过程中碳水化合物及生物碱的积累研究

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关键词陕西石蒜;鳞茎膨大;碳水化合物;生物碱含量;合成关键基因表达

中图分类号 S682. 2+9 文献标识码A

文章编号 0517-6611(2025)13-0094-06

doi: 10.3969/j . issn. 0517-6611.2025. 13.018

开放科学(资源服务)标识码(OSID):

Differential Accumulation of Carbohydrate and Alkaloid During Bulb Development in Lycoris shaanxiensis CUI Dong-jun1 ,GAO Yu2 ,HUANG Shi-yu² et al(1. Anyang Landscape and Greening Research Institute,Anyang,Henan 0;2. Co一 inovationCenterforSustaiableForestryinSouthChia/KeyLaboratoryofStateForestryandCrassandAdminstrationoSubroicalFor estBiodiversityConservation/CollgeofEcologyandEnvironment,NanjingForestry University,Nanjing,Jiangsu20037)

AbstractObective]Indertoinvestgatetechangesincarbhyrateandalkaloidacumulationduringtheulbevelopmentproesof Lycorisshansis,sokillprvidathetialsisfeleatigtethdalaldcculatioofLyoisbod Materialsfrodiferentsagsoflevelopentwereudtoivestigatetangsinubgoth,alodotent,Galostsisat waygeneexpresiocarbohdratecotentandeeactivituringtebubevelopntprocs.Result]Withheevelotofth bulb,thedameeedofllosndtelat. Among the three important alkaloids,galantamine(Gal) had the highest content in S4,reaching 457.31μg/g ,while lycoramine(Lycm)and lycorine(Lyc)adtegstconentiSat826.d65.32/espetiely.GalLyadLcprductioeealstin S4. The expression levels of PAL,C4H,OMT genes were increased as the bulb developed. The overall content of carbohydrates increased with thebulbdevelopment,andteactivitiesoftahstsisezes(AGPase)gaule-bundachsase(GBS)andublesachs thase(SSS)oetaereeleratigsachstsis;tectiiyfaasedtaseisloed byacelerating ebeakdoftarhintosolublsugars.Conclusion]issdyprelinarilyevealedthangesincarbohdrateadal kaloidmetabolisduingthulvelopentprossofLycorisshanesis,bohdratelvelsontiets,hileakalodtet peaksattheS4stag,tisokwillprovdeateoreticalbsisfeleratingthgrowhandaaloidaccuulatioofLorisbbsusg lecular biology and other methods in the future.

KeywordsLycoris shaanxiensis;Bulb development;Carbohydrates;Alkaloid content;Expression of key genes in synthesis

石蒜属(Lycorisspp.)的分布中心在我国,具有较高的观赏和药用价值,现属内约有29个种,我国资源占全球总量的80% 以上[1-4]。(剩余14602字)

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