宁夏枸杞LbDXS基因家族全基因组鉴定及表达分析

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中图分类号：S718.43；S663.9 文献标志码：A 文章编号：1673-923X（2025）08-0154-13

Abstract：【Objective】BasedontheetiregenomeofLycumbarbarum，teidentifcationofthegenefamilyelatedtotesthsisof ZeaxanthindipalmitateamelyDX，ascadouttoaleitsexpressncharacteristicsuringtedevelopentpodfLm barbarumandprovidecandidategenesfortheanalysisof Zeaxanthindipalmitatebiosynthesis.【Method】Utilizingthewhole-genome datandtranscriptomedataofLyciumbarbarum，theLbDXSgenefamilywasidentifiedthroughbioinformaticsmethods.Itssequence characteristicsnestructure，ysicochemicalpoptisofincidsonseedproteidmai，ygeeticelatiosi thefamilytuedstdfret barbarumwereexamined.【Result】Atotalof7LbDXSgenefamilieswereidentifed.Theirsequencecharacteristicsrevealedhatall ofthemcontainedtheconservedstructureofDXSandwereclusteredintofourcategoriesthroughevolutionarytreeanalysis.Among them，thegrouplfamilyhadthelargestnumberofmembers.ThesevenLbDXSgenesweremainlydistributedonthefivechromosome. Themulti-speciesvotionarytrealyisdicatedthattemembersoftesaeamilyereevoutionrilyoseeddit wasspeculatedthattheyhadsimilarfunctionsingeneticevolution.Theanalysisofthepromotercis-actingelementsshowedthathe activationregioofteLDetadespoingtsaedtolitsposeoesoetald forth.ThecolinearanalysisdemonstratedthattheLbDXSgenehadsimilarfunctionsintheevolutionofthesoanaceae.Theexpsion levelsofLbDX2，LbDX3，andLDX4increasedsigniantlyfromthefruitsagetotheipenngsgeuggestingatb2， LbDXS3，andLbDXS4mightplayacrucialrole intheaccumulationof Zeaxanthindipalmitate.【Conclusion】Sevenmembersofthe LbDXSgenefamilywereidentifiedbasedontheentiregenomeofLyciumbarbarum，allofwhichcontainedtheconserveddomain ofDXS.Evolutionaryanalysisspeculatedthattheirfunctions wersimilaramongthespeciesof thesolanaceaefamily.Amongthem， LbDXS2，LbDXS3，andLbDXS4weresignificantlyexpressedduringthestagefromthefruitstage totheipeningstageinLycium barbarum.Theseresultsindicatethat theyhavepotentialregulatoryfunctionsinthebiosynthesisofZeaxanthindipalmitate.

Keywords：Lyciumbarbarum;LbDXSgenefamily，expressionanalysis

茄科Solanaceae枸杞属LyciumL.有80多个物种，主要分布在温带和亚热带地区。（剩余15719字）