基于非靶向代谢组学分析雪岭云杉响应畸形金锈菌侵染的代谢物变化

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中图分类号：S791.248 文献标志码：A 文章编号：1673-923X（2025）08-0132-12

Abstract：【Objective】Utilingnon-targetedmetabolomicstechnologytoinvestigatetheimpactofChysomyxadeformans （Diet.） JacZ.onthemetabolitesofPschrenkiana.Thefindingsaimtoprovideatheoreticalbasisforthepreventionandcontrolofustdisease inP.schrenkiana【ethd】IntissudyurrentearalthnddseasedPsrenianaootsdleaveseccted targetedmetabolomicsstudywasconductedusing liquidchromatography-masspectrometry（LC-MS）technology.Thediferecein metabolites between healthy and diseased P.schrenkiana were compared， thresholdsof P>1.0 ，fold change >1.500 or fold change < 0.667 and P<0.05 were set for screen diferential metabolites and the metabolic pathways were annotated through the KEGG database. Furthemoreepthalysisoftefolateotsisphayteeeidosythsisataydtetyateabls pathwaywasconducted.【Result】SignificantdiferenceswereobservedinthemetabolitesofhealthyanddiseasedP.schrenkiana. Therewere57and418sigfantlydierentmetabolites（DMs）intpositivandegativeiomodesespectivelyeitial metabolicproductsweremainlyannotatedtoenvironmental informationprocessng，gneticinformationprocesing，andetabolic pathways.DataaalysisfroteKEGbubblechartdicatedthatthfolatebosyesispathwaydtepenoidbiosythesisathway andbutyrate metabolismpathwayplayedcrucialroles inthepathogenesis processofthepathogenonthehost.【Conclusion】Rust fungusinfectionsiiicantlyectstemetabolitesofshekinaadmetabolomicsaalysissggststatteathogetriger theocurrenceofPsrenianustiseasethoughangesinajorsecondaryetabolitesinthoatemetabolisathayutyate metabolismpathway，and diterpene biosynthesis pathway.

Keywords：Piceaschrenkiana;rust;non-targeted metabolomics;KEGG

雪岭云杉Piceaschrenkiana是松科Pinaceae云杉属PiceaA.的常绿乔木，在我国分布于天山南北坡、昆仑山西部、准噶尔西部山地，面积约5.28×105hm2 ，占新疆天然林的 44.9%[1] ，东西绵延 1800km ，向西延伸到天山与阿赖山，是中亚荒漠带最主要的山地常绿针叶林。（剩余16149字）