舞毒蛾化学感受蛋白基因 LdCSPs 克隆及表达分析

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中图分类号：S763.42；S433.4 文献标志码：A 文章编号：1673-923X（2025）08-0144-10

Abstract：【Objective】TheaimofthisstudywastocloneLdCSP1andLdCSP2genesofchemicalreceptorproteinsofLymantria dispar，andtostudythechaacteristicsgeexpressonandesstaetodugstressexpressonofdCSPsgnes.histudylaid foundationforrevealingthecharacteristicsofchemicalreceptorproteinsLdCSP1andLdCSP2genesandtheirspecificfunctionsin drugmetabolism，andalsoprovidedanewideaforfiding potentialdrugtarget genesand preventinggypsymoth.【Method】Based onthetranscriptomesequencingresultsofLdispar，twochemicalreceptorproteingenesofLdisparwereconedbyRT-CRORF Finder，ExPASyignalP4.1NCBICDandtheronlinetoolswereusedforoiformaticsanalysis.qR-PCRwasusedtoaalye theexpressionofCSPgenesindiferentspeciesofL.disparanduderrotenonestress.【Result】ThetotallengthofLdCSPgeneORF is360bp，encoding119aminoacids.hetotallengthofLdCSPgneORFis375bpencodng124aminoacids.Sequencealsis showedthattheaminoacidsequencesof thetwoCSPofLdisparhad4conservedcysteineloci，nd wereconsistent withtheCSPs structurepatern“C1-X6-C2-X18-C3-2-C4.PhylogenetictreeanalysisshowedthattwoLdCSPswereclusteredintotwobraches ofotherepidopteraCSPs，andeachLdCSPsequencewasclusteredintoasmallbranchwiththesequencewithhigherhomologyThe expressionofLdCSPgenesatevelopmentstageshowedthatLdCSPgeneswereexpressdinallstagesofL.disparandterelative expressionlevelsofLdCSPandLdCSP2genes werethe highestathethirdstage （I3D1），andthelowestattheeggstage.Tissue expression showed that LdCSPs wereexpressed in different tissues of L disparatthe thirdinstarlarvaestage. LdCSPs genes had the highest expression in the thorax，and the tissue expression of the two LdCSPs was different.QRT-PCR results showed that the expression ofLdCSPsgeneinththidinstarlarvaeofL.disparwasfstdecreasedandthenup-regulatedunderrotenoestres.【Concusion】Two Chemosensory proteingenes，LdCSP1andLdCPS2，wereclonedfromL.dispar.QRT-PCR showed thatLdCSPs gene expressionhad timeand dose effects on rotenone stress，indicatingthat LdCSPs playedan important role in themechanism of detoxification resistance ofL.dispar，whichprovidedareferenceforfurtherstudyofthefunctionofthesedrugtargetgenes，ndprovidedanewideaforfuture preventionand control ofL.dispar.

Keywords：Lymantria dispar;chemosensory proteins;gene cloning;expression analysis

舞毒蛾Lymantriadispar是一种分布地域较广的农林食叶害虫，具有周期性发生特点。（剩余16809字）