红松PkSERKs基因克隆及在不同类型愈伤组织中的表达分析

打开文本图片集

中图分类号：S722；Q943 文献标志码：A 文章编号：1673-923X（2025）08-0001-11

Abstract：【Objective】This studyclonedthePkSERK genes from Korean pine（Pinus koraiensis）andanalyzedtheir expresion characteristics，layingafoundationforfunctionalresearchonPkSERKsinsomaticembryogenesisofKoreanpine.【Method】Tefullengthcodingsquence（CD）ofERK-3eneinKoreanpineassuessfullloed，subsequentbifomaticsalysere conducted toexaminetheproteinstructure，pyicochemicalpropetis，andevolutionaryelationships，subcellarlocalationwas observedithotetalldps koreanpinecalliwereanalyedy-CRtechnology【Result】SERK1，SERK2，andSERK3weresuccessullyod，i cDNAlengthsof1824，1887and1437bp，encoding 607，628and448amnoacids，respectively.PkSERK1isanacidic，hdohlic andstableprotein，hileERK2isanacidic，hydrophilic，andustableprotein，ndPERK3isbasic，hydrophilicndstable protein;bothPkSERKlandPkSERK2containasignalpeptide，whereasPSERK3doesnothaveasignalpeptide;tesetrproteins arealltransmembraneproteins.ThePkERKlproteinhastheclosestgeneticrelationshipwithCsERK1-3ofCuninghamialanceolata，

PkSERK2hastheclosestgeneticrelatioshipwithCsSERKlofCameliasinensisandPkSERK3hastheclosestgeneticrelatiship with CjSERK3ofCryptomeriajaponica.PkSERK1andPkSERK2werelocatedinthecellmembrane，whilePkSERK3waslocatedin thecellmembraneandnucleus.TePkERK1geneashighlyexpresedinbothcalluswithighembryogenicpotentialandcaluswith globularembryosofkoreanpie;PkSERK2washighlyexpressedinbrowncalls;PkSERK3wasonlyhighlyexpressdincallus with highembryogenicpotential.【Conclusion】TegenesofPkSERK1，PkSERK2andPkSERK3wereclonedfromkoreanpine，PkERK1 mayregulatethemaintenanceofembryogenicpotentialincallusandearlyglobularembryodiferentiationinKoreanpine，high expresionofPkSERK2maybeassciatedwithbrowningembryogeniccalus（EC）andearlyglobularembryodiferentiationinKorean pine，while PkSERK3 may onlyplaya role in the maintenance of embryogenic potential in Korean pine callus.

eywords：Pinus koraiensis;somatic embryogenesis;embryoniccallus;PkSERKs;gene cloning; expressionanalysi

红松Pinuskoraiensis是寒温带针阔叶混交林的主要建群树种，也是中国乃至东亚地区极其重要的优质珍贵用材与坚果树种。（剩余18126字）