陆地棉苗期耐低氮种质鉴评与筛选

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中图分类号：S562 文献标志码：A 文章编号：1008-0864（2025）11-0102-09

Abstract：In order to analyze the nitrogen deficiency tolerance of coton germplasm resources，in pots with low nitrogen （15 g⋅m-2 ）and normal nitrogen（ 45g⋅m-2 ）conditions under a rainproof shelter at the seedling stage，root dryweight，shoot dryweight，totaldryweight，ratioofroot toshootandchlorophyllvalueof419 uplandcotton germplasm resources were measured，and their nitrogen deficiency tolerance indexes were calculated.Through correlation analysis，principal component analysis and membership function analysis，the comprehensive evaluation of nitrogen deficiency tolerance of 419 coton germplasm resources was conducted.The results showed that，under lownitrogen stress，therewas extremely significantly negative correlation between thenitrogenresistance index in shootdry weightand inratioofrootto shootofcotton seedlings.However，other traits showed significantlypositive corelations or extremelysignificantlypositive correlations.Principal component analysisclasified 5 indexes into 3principal components，whose cumulative variance contribution rate was 98.590% ： D -valueswerecalculatedbased onthe weightsofthese3 principalcomponents.Then the419 germplasms were divided into4 gradesand10 nitrogen deficiency tolerant germplasms were screened.Furthermore，through stepwise regresson analysis，aregression equation was established as D=-0.123+0.167XRDWNRI+0.252XTDWNRI+0.132XSPADNRI （ XRDWNRI ， XTDWNRI and XspapNRI were nitrogenresistance indexesofrootdryweight，totaldryweightand chlorophyllvalue）.Aboveresultslaid foundation for further mining of nitrogen deficiency tolerant genes and improving nitrogen deficiency tolerance of cotton.

Keywords：upland cotton；core collction；seedling stage；nitrogen deficiency tolerance；screening

氮（nitrogen，N）是维持植物生命活动的必需养分，是核苷酸、氨基酸、蛋白质、叶绿素、激素和维生素等许多重要物质的组成成分，对作物的生长发育和产量形成起重要作用。（剩余15091字）