小麦苗期外施脯氨酸增强抗旱性的生理机制研究
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中图分类号：S512.1 文献标志码：A 文章编号：1008-0864（2025）11-0093-09
Abstract：Inorder to investigate the efects of proline（Pro）applicationon seedling growth of wheat under drought stress，wheat varietyBainong2O7 wasusedasexperimental material，and4 treatmentswereset，including water （CK），20% PEG-6000 solution（PEG），20 mmol·L-1 proline（PRO）and 20% PEG-6000 solution+20 mmol·L-1 proline（PFG + PRO）.The physiological mechanism of drought resistance in wheat treated with proline at seedling stage were analyzed.The results showed that，before drought stress，there were no significant differences in SPAD value and thecontents of malondialdehyde（MDA），soluble sugar（SS）and Pro among 4 treatments.After drought stress，compared with CK，SPAD value of PEG treatment was decreased，the contents of MDA，SS，Pro were increased，and relative expressions of SOD ， and P5CS genesincreased，seedling height，stemand leaf fresh weight，stemandleafdryweightandrotdryweightinPEG treatment weresignificantlylower，androottoshootratio was significantly higher.Compared with PEG treatment，the SPAD valueand the contents of SS，Pro were significantlyincreased，MDAcontentsignificantlydecreased.After1dofdroughtstress，therelativeexpressionsof SOD ， and CAT genes in PEG + PRO treatment were significantly higher than thosein PEG treatment.After2 d of drought stress，the relative expressions of LEA and P5CS genes were significantly higher than those in PEG treatment.After drought stress，the plant height，stemand leaf dry weight of PEG + Protreatment were significantly increased by 11.64% and 12.96% compared with PEG treatment.In conclusion，external application of Pro at seedling stage could reduce MDA contentin leaves，increase SPAD value，the contents of SS，Proandthe relative expression levels of stress-related genes including SOD，POD，CAT，LEA and P5CS ，which could improve drought resistance of wheat and layafoundation for normal growth and development of wheat in thefollwing stage.
Keywords：winter wheat；exogenous proline；drought stress；relative gene expression
小麦作为世界三大粮食作物之一，其优良品质对人类生活水平的提高具有不可或缺的作用，小麦产量的高低对粮食安全也有很大影响。（剩余17372字）
