TRV介导的上海青和芥菜VIGS体系的构建

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doi：10.13304/j.nykjdb.2025.0124

中图分类号：S63 文献标志码：A 文章编号：1008-0864（2025）08-0239-11

Constructionof TRV-mediated VIGS System in Brassica rapa subsp. chinensis and Brassica juncea

MA Yanqin 1，2 ，ZHOU Yujie1²，LONGHaicheng1，²，LI Ju1，²，WANG Haie 1，2 ， CHANG Wei³， LI Zhi 1，2 ， ZHONG Jian1，2， MIAO Mingjun 1，2. ， YANG Liang1，2*

1.VegetableGermplasmInnovationandVarietyImprovementKeyLaboratoryofSichuanProvince，KeyLaboratoryofHorticultural CropsBiologyandGermplasmEnhancementinSouthwestRegionsofMinistryofAgricultureandRuralAairs，Horticulture Research Institute，Sichuan Academyof AgriculturalSciences，Chengdu 61oo66，China;2.Sichuan Province Enginering TechnologyResearchCenterofVegetables，SichuanPengzhou611934，China; 3.Sichuan Institute of Edible Fungi，Chengdu 61Oo66，China）

Abstract：To establishan eficientvirus-induced gene silencing（VIGS）system in important Brasicaceae vegetables，Brassca rapa subsp.chinensisand Brassica juncea were chosenas the material and theendogenous phytoene desaturase （ PDS ）gene was selected as the marker gene.The pTRV2-BrPDS，pTRV2-BjuPDS- and pTRV2-BjuPDS- c vectors were constructed，and the infection efciency of Agrobacterium was optimized to construst VIGS system with high efficient.The results showed that the pTRV2-BrPDS，pTRV2-BjuPDS- g and pTRV2-BjuPDS（204号 c vectorswere successfully constructed.The albinism phenotypeof B.rapa subsp.chinensis plants was the most obviouswith OD 600 =1.0 of Agrobacterium，while the silencing efficiency ofB.juncea wasoptimal with OD 600 =0.8of Agrobacterium.TheqRT-PCRanalysisconfirmedthattheobserved albinismphenotype wasresulted fromthesilencing of theendogenousPDSgene inplant infectedwith the TRV-basedrecombinant virus.Aboveresultsprovided theoretical basis for studing the gene functions of B.rapa subsp.chinensis and B. juncea using VIGS technology. Keywords： Brassica rapa subsp. Chinensis； Brassica juncea；VIGS；PDS gene;albinism phenotype

病毒诱导的基因沉默技术（virus-inducedgenesilencing，VIGS）是一种高效验证植物基因功能的手段。（剩余15124字）