棉花GhDMT7基因对5-氮杂胞嘧啶核苷的响应机制

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doi：10.13304/j.nykjdb.2024.0522

中图分类号：S56 文献标志码：A 文章编号：1008-0864（2025）08-0028-08

Response Mechanism of Cotton GhDMT7 Gene to 5-azacytidine

YANG Zhining1，²，LUXuke 2，3 ，FANYapeng²，SUNYuping²，YUXin²，WANGLiang4， GAO Yongjian4，Gulijiayinashen Habuli4，Gulishaxi Nasiyi4，Wumuer Abudu4，HUANG Hui²，

ZHANG Menghao 2 ，WANGLidong²，CHENXiao²，XIAO Lei²，ZHANG Xinrui²，WANG Shuai 2，3 CHEN Xiugui 2，3 ，WANG Junjuan²，GUO Lixue²，GAO Wenweil*，YE Wuwei1，2.3*

（1.CotonEngineeingResearch CenterofMinistryofEducation，CollgeofAgriculture，XinjiangAgriculturalUniversity，Urumqi

830052，China；StateKeyLaboratoryofotoBbreeingandIntegratedUtlzation，InstituteofCottonResearchCine

AcademyofAgriculturalSciences，HenanAnang4o，China；3.NationalCenterofTechnologyInnovationforComprsive UtilizationofSaline-alkaliLand，ShandongDongying257ooo，China;4.AgriculturalTechnologyExtensionCenterof Shawan City，Xinjiang Shawan 8321OO，China）

Abstract：To investigate the effects of 5-azacytidine（5-azaC）onthe expresson of the GhDMT7 gene andseed germinationofcotton（Gossypium hirsutum L.），this paper analyzed the expression level of GhDMT7and the growth traits of coton seed.TheGhDMT7genewas clonedand performedcomprehensivebioinformaticsanalysis.Theresults showed that GhDMT7 protein was astable hydrophilic protein with no signal peptide and transmembrane domains. Undersalt stress conditions，the malondialdehyde content incotton leaves increased，indicating exacerbated membrane damage.5-azaC exhibited a dosage efect on GhDMT7 expression with slightly up-regulated expression at low dosage and down-regulated expression at high dosage，and decreased 5-methylcytosine （ 5mC ）content.

Furthermore，the treatment with 50μmol⋅L-1 5-azaC significantly enhanced seed germination potential and growth parameters，which promoted seed germination.Above results laid a foundation for further studying the function of GhDMT7 on coton growthand development，and provided genetic resources forbreeding new varieties of salineresistant cotton.

Keywords：Gossypium hirsutum; DNA methyl transferase；gene cloning; bioinformatics analysis；qRT-PCR

棉花（Gossypiumhirsutum L. ）是重要的经济和纤维作物，也是盐渍地的先锋作物。（剩余13542字）