一株拮抗葡萄灰霉病菌的贝莱斯芽孢杆菌筛选及鉴定

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doi：10.13304/j.nykjdb.2024.0386

中图分类号：S182 文献标志码：A 文章编号：1008-0864（2025）08-0110-09

Screeningand Identificationofa Strain of Bacillusvelezensis AgainstBotrytiscinerea

LIHaiyan 1 ，ZHANGTing1，LIXinchang1，ZHANGLing1，WANGPei²，BAOMinhu1* （1.ChengdeAcademyofAgriculturalandForestrySciences，HebeiChengdeO67ooo，China;2.NationalAgro-techExtensionad Service Center，Beijing100125，China）

Abstract：InordertoisolateabiocontrolbacteriawithantagonsticfetsonBotrtiscinerea，theantagonisticbacteriaere screned from graperhizosphere soilsbyplateconfrontation methodandliter paper method.Astrain with strong antagonistic efectwasselectedandidentifiedbymorpholoigical，physiologicalandbiochemicalcharacteristics，16SrDNAsequences analysis.Thesafety，growth-promotingand thecontrol efectof thestrain were testedbytheblood platemethod，seed germination experimentsanddetached fruitmethod.Theresultsshowedthat5antagonisticbacteriawerescreened from142 bacterialstrainsisolatedfromgraperhizospheresoils.These5antagonisticbacteriahaddiferentinhibitoryefectongrape gray mold disease with the inhibition rates from 81.74% to 89.13% ，and the inhibition zone were from 2.42 to 11.08 mm， amongwhichtheinhibitoryeffectof strainB1-4wasthehighest.Bymorph-oloigical，physiologicalandbiochemical characteristics，16SrDNA sequences，strain B1-4 was identifiedasBacilusvelezensis，which hadno hemolysis，could producecelulase，proteaseand siderophores，and hadnitrogen fixation efect.Thegerminationrateandradiclelengthof cucumberandrapeseed seedscould besignificantly improved by10O times dilutionofB1-4fermentationsolution.The protective effect of B1-4 fermentation liquid reached 70.31% and the rapeutic effect was 69.53% on postharvest grape fruit gray mold，which was no significant diference with 5 OoO times dilution of 50% fludioxonil （ 71.88% ）.Above results showed thatB. velezensis B1-4 was a protential biocontrol strain for control B. cinerea.

KeyWords：Bacilusvelezensis；Botrytiscinerea；isolationandidentification；growthpromotion；antagonisticeffect

灰霉病是对葡萄生产具有重要影响的病害之一，病原菌为灰葡萄孢（BotrytiscinereaPers.）]，该菌可侵染葡萄的叶片、花穗、果穗和穗轴等，破坏植物组织正常生长，严重影响葡萄品质和产量[2]。（剩余15603字）