板栗R2R3-MYB基因家族的鉴定及表达分析

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doi：10.13304/j.nykjdb.2024.0477

中图分类号：S664.2，Q344+.13 文献标志码：A 文章编号：1008-0864（2025）08-0047-13

Identification and Expression Analysisof R2R3-MYB Gene Family in Chinese Chestnut

LIU Yaming§，YANG Yong§，KANG Xiaoxiao，WANG Meng，WANG Dongsheng， ZHANG Haie*

（Hebei ProvinceChestnutIndustryCollborative InovationCenter，Engineering Research CenterofChineseChestnutIndustry TechnologyofMinistryofEducation，Colegeof HorticulturalScienceand Technology，HebeiNormal Universityof Science and Technology，Hebei Qinhuangdao O66Oo4，China）

Abstract：R2R3-MYB transcription factorsare the largest MYB subfamily inplantsandare widelyinvolved inregulating plant growth and development and stress response.R2R3-MYB gene family of Chinese chestnut was genome-widely identified，andthe chromosomedistributionof these genes，subcelular localization prediction，phylogeneticrelationships， proteinmotif，gene structureandsequencealignmentofconserved domains wereanalyzed.Finally，the expresionof the CmR2R3-MYB gene familyunder drought stresswas analyzed by transcriptomeand RT-qPCR.The results showed thattotal 141 CmR2R3-MYB genes were unequall distributed on12chromosomes and1 contig fragment.And the phylogenetic tree divided theminto6groups.There were7CmR2R3-MYB genes diferentiallyexpressedunderdroughtstress.Among them， CmMYB89 was upregulated，while CmMYB32，CmMYB95，CmMYB91，CmMYB119，CmMYB131andCmMYB104 were downregulated，which indicatedthatthese7genescouldbe involvedindroughtresistanceofChinesechestnut.Aboveresults provided theoretical foundationsfor furtherexploring theroleoftheR2R3-MYBgenefamilyinresponse todroughtstresof Chinese chestnut.

Keywords：Chinese chestnut；drought；R2R3-MYB gene family

转录因子是一类能够与启动子区域中顺式作用元件发生特异性结合的蛋白质分子，具有调节mRNA表达的功能，在植物生长发育和胁迫响应中发挥重要作用[2-3]。（剩余15152字）