拟南芥AtCHX19基因在盐碱胁迫应答中的功能解析

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doi：10.13304/j.nykjdb.2024.0214

中图分类号：Q786 文献标志码：A 文章编号：1008-0864（2025）08-0060-13

Functional Analysis of Arabidopsis thaliana AtCHX19 Gene in Response to Salt-alkali Stress

， ， ， ， ， Bowei*，SUNMingzhe* （Crop Stress Molecular Biology Laboratory，Collge of Agriculture，Heilongjiang Bayi Agriculture University， HeilongjiangDaqing ，China）

Abstract：Cation/H+exchanger（CHX）gene family participatein theregulation of plants proton and cation transport， and playan importantrolein plant growthanddevelopmentandabioticstress response.ToanalyzetheArabidopsis AtCHX genes in response to salt-alkali stress， 28AtCHX genes of Arabidopsis were analyzed.The results showed that AtCHX genes were divided into5subfamilies，and their structuresand protein sequence motifs were very conserved. Tissue expression and abiotic stress expression analysis found that AtCHXIq expressed in roots of Arabidopsis and upregulatedbysalt-alkali stress.Furthersalt-alkali toleranceanalysisofArabidopsis mutantshowed thatthedeletionof AtCHX19 gene reduced tolerance to high saltand soda saline stress，andNa+content of mutant was significantly higherthanthatof wildtype.Aboveresults initiallyclarified thattheAtCHX19 generesponded tosaline-alkali stress byregulating Na*transport，which provided data support for further elucidating the stressresistant molecular mechanism and signaling transduction pathway mediating by CHX.

Keywords：AtCHX19；saline-alkali stress；functional analysis;CHX

植物生长发育受环境变化影响，其中土壤盐碱化是制约植物正常生长发育的环境因素之一。（剩余19723字）