水稻生长调节因子GRF基因家族的鉴定及生物信息学分析

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doi：10.13304/j.nykjdb.2024.0328

中图分类号：S511，Q781 文献标志码：A 文章编号：1008-0864（2025）08-0018-10

Identification and Bioinformatics Analysis of Growth Regulating FactorGRFGeneFamily inRice

LIU Zhien，HEYong，WANG Zhicheng，ZHANXiaokang，WANG Tingbao， LIU Yaowei，TIAN Zhihong （College of Life Science，Yangtze University，Hubei Jingzhou 434025，China）

Abstract：Asa plant-specific transcription factor，growth regulatingfactor（GRF）plays an important rolein plant growth and development.ToidentifythemembersofthericeGRFgene family，proteinphysicochemical properties，sequence comparison，secondaryand tertiarystructureprediction，subcellularlocalisation，genestructure，cis-acting elements， chromosomallocalisation，evolutionary treeconstructionand covariance were analyzedusing the bioinformatics methods.The results showedthat12GRF genes were identified.Thelengthofrice GRFproteins was 211～456aa，the molecularweight was 22.3～49.3 kD，theisoelectric point was 4.7～9.85，and the subcelularlocalisation was mainly located inthenucleus.The rice GRF genes contained 2～5 exons，and the promoter elements were mainly relatedto hormone regulation，stress response and light response.12 GRF genes were unevenly distributed on chromosomes 2，3，4，6，7，11 and12.GRFgenes in rice werecloselyrelatedtomaize，andhadlowhomologywithtomatoandArabidopsis.There wereno tandemduplicationevents in the rice GRF genes，and large segmental duplications were the main expansion modes.Above results provided a theoretical basis for the in-depth study of rice GRF gene function.

Keywords：rice；growth regulating factor;GRF gene family;bioinformatics

转录因子（transcriptionfactor，TF）也叫反式作用因子，是一种特殊的蛋白质，其能与基因上游的启动子序列结合，在其他蛋白的共同参与下能够抑制或激活转录，在植物生长发育、果实成熟、衰老、应答和适应各种胁迫以及防御反应中起重要作用[2-3]。（剩余12054字）