β-甘露聚糖酶的性质、热稳定性改造及应用研究进展

打开文本图片集

中图分类号：Q55 文献标志码：A

Research progress on properties， thermostability modification，and application of β -mannanase

GAO Xiaopeng 1 ，GUAN Fuhao1 ，GUO Chao²，WANG Chao² ，LIU Yuchun 2 （1.College of Life Science，Yan'an Univercity，Yan'an 716ooo，Shaanxi，China; 2. Academy of National Food and Strategic Reserves Administration，Beijing lOoo37，China）

Abstract We investigate the properties，thermal stability，and industrial applications of β -mannanase，an endoglucanase that hydrolyzes β-1 ， 4 -mannosidic linkages. It is widely used in feed， food processing，textile industries，and biofuels. We first summarize the basic characteristics of β -mannanase，including its distribution in animals， plants，and microorganisms，as well as the optimal reaction conditions and stability of enzymes from different sources. Subsequently，we analyze the structural factors affecting the thermal stability of β -mannanase，such as metal ion-binding domains，carbohydrate-binding domains， flexible regions，and salt bridges，and propose molecular engineering strategies to enhance its stability，which is crucial for its industrial applications. We also discuss the industrial applications of β -mannanase， particularly as a prebiotic in feed，and its potential uses in food processing，detergent，and textile industries， highlighting its multifunctionality and impact on increasing industry efciency and sustainability. Finally，we anticipate future research directions， including the exploration of new enzyme resources，optimization of structural modification strategies，and balancing the thermal stability and catalytic activity of enzymes.

Keywords β -mannanase; thermal stability; molecular modification； enzymatic properties； ra-tional desin

0引言

β -甘露聚糖酶是一类能够水解β-1，4-D-甘露糖糖苷键的内切糖苷水解酶（glycosidehydrolase，GH）。（剩余22662字）