生物炭基菌剂的制备及应用效果研究

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中图分类号：TQ446.3 文献标志码：A DOI：10.13338/j.issn.1674-649x.2025.01.004

Research on the preparation and application effect of biochar-based bacterial agent

LIUYueqin¹，MA Quan1，CHEN Feng1，LIU Xiaochen 2 （2号

（1.College of Life Science，Yan'an University，Yan'an 7l6ooo，Shaanxi，China; 2.School of Environmental and Chemical Engineering，Xi'an Polytechnic University，Xi'an 710048，China）

AbstractIn order to further improve the effectiveness of phosphorus bacteria agents，phosphate solubilizing bacteria （PSB） biochar-based microbial agents were prepared by adding diferent amounts of corn stover biochar （CSB） in PSB solution. The preparation conditions of the biochar-based microbial agents were analyzed using OD600 and effective viable bacterial count as indicators. Through pot experiments，the effect of biochar-based microbial agent on wheat growth performance and soil physicochemical properties were investigated. The results showed that when the addition amount of biochar was 0.16% ，the OD600 of the prepared biochar-based microbial agent significantly increased，and the effective viable cell count reached 8.37 CFU/pL，which was 3.3 times higher than the CK1（without CSB） group. Biochar-based microbial agent T group （CSB+PSB） could effectively promote the growth of wheat after four weeks of application，the plant height，chlorophyll content，and root activity increased by （10，25±0.62）% ，（67.69 ±4.21）% ，and （49.58±3.73）% ，respectively，compared to the CKl group. At the same time，biocharbased microbial agents effectively improved soil properties，with soil available nitrogen，soil available phosphorus，soil catalase，and urease activities increasing by （31.81±2.25）% ， （50，71±4.58）% ，（30.89 （204 ±1.43% ，and （44.26±3.84）% ，respectively，compared to the CKl group. The CK2 （with CSB） group treated with biochar alone showed slightly higher indicators than the CKl group. Adding a certain amount of biochar had a certain effect on improving wheat growth and soil properties，but the phosphorus solubilizing bacteria biochar-based microbial agent prepared with biochar as a carrier could more effectively improve soil fertility and enhance wheat growth promotion performance，providing a theoretical basis for further research and development of microbial agents.

Keywordsbiochar；phosphate-solubilizing bacteria； biochar-based microbial agent； soil perfor-manc;growth-promoting properties

0引言

我国是农业废弃物产生数量最多的国家[]，对固废资源的处理方法主要是肥料化和能源化利用或就地燃烧[2]。（剩余12206字）