咖啡酸苯乙酯生物合成体系的构建及优化

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中图分类号： 文献标志码：ADOI：10.13338/j.issn. 1674-649x. 2025.01.002

Construction and optimization of Caffeic acid phenethyl ester biosynthesis pathway

ZHANG Genlin，LI Yuanyuan，QIU Di，ZHAO JinYu （College of Chemistry and Chemical Engineering/Key Laboratory of Chemical Green Processing of Xinjiang Corps， Shihezi University，Shihezi 832Oo3，Xinjiang，China）

Abstract Caffeic acid phenethyl ester （CAPE） has various pharmacological activities； however， its natural sources are limited，and chemical synthesis poses environmental hazards.Its microbial synthesis using synthetic biotechnology will be more in line with green economy. In this study， CAPE was synthesized in yeast by constructing its de novo synthesis pathway，overexpressing acyltransferase，optimizing 2-phenylethanol pathway and phenylpyruvate pathway. After 120h of fermentation，the titer of it reached （98.56±4.56）μg/L by yeast engineering strain S4. After the balancing of the supply of two precursors，caffeoyl coenzyme A and 2- phenylethanol，a caffeoyl CoA synthesis engineering strain was consrtruted through expressing tyrosine deaminase （TAL），hydroxycinnamoyl transferase（HCT） and CoA ligase （4CL） in Escherichia coli. Then， a mixed culture system was constructed with yeast engineering strain S4 and caffeoyl CoA synthesis engineering strain，resulted in（667.64±10.26）μg/L of CAPE after optimizing the culture conditions. This study provides a reference for the cellular engineering synthesis of CAPE.

Keywords caffeic acid phenylethyl ester; path optimization; biosynthesis;mixed fermentation; 2-phenylethanol

0引言

咖啡酸苯乙酯是一种天然酚类化合物，具有抗炎[]、抗氧化[2]、抗病毒[3]、抗癌[4]、抗糖尿病[5、抗白血病和神经保护作用等多种药理活性。（剩余13440字）