鸡白痢抗体检测方式的改良

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中图分类号：S852.61 文献标识码：A 文章编码：1005-8567（2025）03-0105-06

Abstract：To investigate the impactof cryopreservation conditionson the detection of antibodiesagainst Salmonella pullorum and the feasibility of using residual plasma after avian leukosis detection for pulorum disease detection，this study selected386O three-yellowchickens for pullorum disease testing inbatches.Thedetection results using diferent anticoagulants，cryopreserved blood samples，serum，and residual plasma were compared with the standard on-site whole blood plateagglutination detection.The results showed that the positive detection rates of

lithiumheparinanticoagulated blood，ethylenediaminetetraaceticacid dipotassumsalt（EDTAdipotassumsalt， EDTA-2K）anticoagulated bloodand residual plasma were highlyconsistent with theon-site whole blood plate agglutinationdetectionresults.The positive individuals detectedbytheon-sitewhole blood plateagglutination method could becompletelydetected inboth cryopreservedandnon-cryopreservedserumand residual plasma samples.The positive detectionrateofserumsamples was slightlyhigher thanthestandard method，andshort-termcryopreservation had no significantadverse effctonthedetectionresults.Inaddition，theactualapplication efectof using residual plasma for pullorum purification was evident.

Keywords：Chicken dysenterydetection；Plasma residual fluid;Frozen conditions

鸡白痢沙门氏菌（SalmonellaPullorum，S.Pullorum）是一种严重危害家禽健康的病原菌，对鸡群危害较大，受感染的雏鸡急性发病后死亡率较高，即使鸡群耐受后，其生长也受到影响，给养殖业带来严重经济损失[1]。（剩余8005字）