经典型和新型鹅呼肠孤病毒的双重RT-qPCR检测方法的建立和应用

打开文本图片集

关键词：鹅呼肠孤病毒；GRV和NGRV；染料法；RT-qRCR;熔解曲线中图分类号： S852.65+91 文献标识码：A 文章编码：1005-8567（2025）03-0096-09

Abstract：The Goose reovirus（GRV）comprises two distinct types：the classic（GRV）and the novel goose reovirus（NGRV）.These two types exhibit diferences in theirepidemiological characteristicsandviral gene sequences.Thelack ofsensitiveandspecificassays forboth typesof viruses hasresulted indificultiesinaccurately diagnosing and timely intervening incases.Inorderto distinguish GRVfrom NGRV，adual RT-qPCR assy based on a dye-based method（SYBR Green I）was established.The results demonstrated that the method was capable of distinguishing GRV（ 79.5∘C ）and NGRV（ 86.5qC ）based on their respective melting curves.Furthermore，no amplification was observed for any of the common waterfowl epidemics，the lowest detections were 6.466 × 101 copies/ （204号 μL GRV and 4.979×101 copies /μL NGRV. The results demonstrated that the dual RT-qPCR assay，based on a dyebased method，couldrapidlyandaccurately identifydifferent types of GRVs in infected geese.Theassay exhibiteda high degree of precision，with coefficients of variation less than 0.55% for both intra-and intergroups.This method has the potentialtosupport earlydiagnosis，epidemiological monitoring and efective preventionandcontrolofthedisease.

Keywords： Goose reovirus;GRV and NGRV;dye-based method;RT-qPCR;Melting curve

禽呼肠孤病毒是呼肠孤病毒科（Reoviridae）刺突呼肠孤病毒亚科（Spinareovirinae）正呼肠孤病毒属（Orthoreovirus）的成员之一。（剩余10129字）