罗伊氏菌素对猪肠上皮细胞免疫调控研究及其在仔猪健康中的潜在应用

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中图分类号：S816.73 文献标识码：A 文章编码：1005-8567（2025）03-0053-10

Abstract：The weaning stage of piglets is confronted with challenges such as imperfect intestinal development， unbalanced microbial community，andan immature immune system，resulting in frequent intestinal diseasesand severelyafecting growthperformance.Thesearch foralternatives toantibioticsisurgent.Inthisstudy，Lactobacills reuteriLR1strainwasisolatedfromthefecesofhealthyweanedpiglets，anditsbiologicalcharacteristicswere analyzed，andtheefectofits metabolitereuterinonporcineintestinal epithelialcells（IPEC-J2）was explored.The results showed thattheLR1 strain grew bestat39.25Cand pH=6.387；reuterinhadsignificant antibacterial activity againstETEC K88，and the inhibitory efect increased with the increase of concentration；it hada time-and concentration-dependent effectonthesurvivalrateof IPEC-J2cell，withcytotoxicityintheearly stageandan increase in survival ratein the laterstage；different concentrationsof reuterin（2 ，20 μL/mL ， 50μL/mL ）

inducedthedifferentialexpressionof interleukinsand chemokines in IPEC-J2cels.This studyprovidesatheoretical basis fortheapplicationofreuterinasanantibiotic-free feedaditiveinpigletbreedingand helps topromotethegreen and healthy development of the livestock industry.

Keywords：Reuterin；Porcine intestinal epithelial cells；Immune regulation；Probiotic metabolite

在现代畜牧业中，仔猪断奶阶段至关重要，同时也面临诸多挑战。（剩余12134字）