锰离子胁迫下解淀粉芽孢杆菌A-3代谢响应

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关键词： Mn2+ ；解淀粉芽孢杆菌；非靶向代谢组学；代谢物中图分类号：S182 文献标志码：A 文章编号：1672-2043（2025）05-1276-10 doi：10.11654/jaes.2024-0522

Abstract：In order to study the metabolic response of Bacillus amyloliquefaciens A-3 to Mn2+ stress， a sterile fermentation broth of A-3 was used as the research object. All metabolites of A-3 were identified by non-targeted metabolomics technology by adding Mn2+ . According to the importance of variable weights（VIP>1）， the differentially expressed metabolites were screened by T-test（ P<0.05 ）， and the A-3 metabolic pathway was enriched by KEGG database. The results showed that Mn2+ stress had a significant effect on the changes of metabolites in A-3 fermentation broth. A total of 332 differential metabolites were identified， 175 were detected in positive ion mode and 157 were detected in the negative ion mode. Among them， 114 metabolites were up-regulated， such as 3- methylindolepyruvate， spermine， adenosine and so on. Down regulation of 218， such as 5-hydroxylysine， citric acid， homogeneous acid and other small analytical organic acids. Through KEGG enrichment and topological analysis of metabolic pathways， 22 metabolic pathways with P<0.05 were found， among which the metabolic pathways of alanine， aspartate and glutamate were most significantly affected. The addition of Mn2+ changes the metabolic level of A-3 cells， among which the expression of spermine is up-regulated. Spermine was involved in glutathione metabolism， pantothenate and CoA biosynthesis and other important metabolic and biosynthetic pathways. A-3 mainly responded to Mn2+ stress by regulating the expression of amino acids， nucleosides and small molecular organic acids.

Keywords：manganese ion; Bacillus amyloliticus; untargeted metabolomics; metabolite

锰元素是地壳中第二大过渡元素，广泛存在于土壤矿物之中，是植物生长必需的微量元素，也是多种酶的重要组成部分，土壤中的锰以多种形态存在，而植物有效态锰以还原型二价的水溶性 Mn2+ 为 主[1-3]。（剩余17185字）