镉修复微生物菌剂的构建及其作用效果

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关键词：植物提取修复；油菜；重金属；Cd修复菌剂；植物-微生物互作；抗氧化系统号：X17；X53 文献标志码：A 文章编号：1672-2043（2025）05-1266-10 doi：10.11654/jaes.2024-0569

Abstract：To elucidate the constructing principles of efficient Cd-remediation agents and its functional effects on oilseed rape， five PGPB strains isolated from the hyperaccumulating plant Sedum alfredii Hance were selected in this study， and the optimal mixed strain combinations were thereby determined by strain co-culture test， and combine it with the enrichment plant oilseed rape， to establish a plantmicrobial remediation system. After that， the effects of Cd-remediation agents inoculation with different concentration ratios on plant biomass， root development parameters， Cd uptake and accumulation， peroxide（ H22 ） contents， glutathione（GSH） contents and antioxidative enzymatic activities were investigated. The results showed that the combination of the two PGPB strains， namely Bacillus sp. Bac1 and Burkholderia sp. Bac2 had the best co-culture growth condition and was more suitable as a Cd remediation agent. In addition， the functional effects of Cd-remediation agents on oilseed rape growth， Cd uptake， and accumulation varied due to the different inoculating concentration ratios of Cd-remediation agents. When the concentration ratio of Bac1 and Bac2 was 3：1 ， inoculation of Cd-remediation agents was more favorable to the growth and development of oilseed rape and the Cd phytoextraction， while the antioxidant system of oilseed rape including antioxidative enzymatic activities， H22 contents， and GSH contents responded more strongly to Cd stress when the concentration ratio of Bac1 and Bac2 was 1∶1.

Keywords：phytoextraction; oilseed rape; heavy metals; Cd-remediation agents; plant-microbe interaction; anti-oxidative system

植物促生菌指不会对植物造成损害，反而可以对植物的生长发育产生正向影响的一些细菌，通常存在于植物根际土壤或植物体内[1-3]。（剩余20156字）