硫化氢信号分子介导S-烯丙基-L-半胱氨酸调控水稻镉胁迫

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中图分类号：S511；X173；X53 文献标志码：A 文章编号：1672-2043（2025）05-1160-09 doi：10.11654/jaes.2024-0744

Abstract：Cadmium（Cd）pollution in paddy soil not only affects the normal growth and development of rice， but also brings potential harm to human health when Cd accumulates in grain. As an important signal molecule， hydrogen sulfide（ H2S ）plays an important role in the regulation of heavy metal stress in plants. In this study， a hydroponic experiment was used to investigate the potential mechanism of Sallyl- ⋅L- cysteine（SAC）， a garlic extract with H2S donor function， in regulating Cd stress in rice. The main rice variety“ZhongZao 35” planted in southern China was selected as the experimental material and sprayed with SAC at series concentration. The results showed that the H2S content in shoots and roots of rice seedlings continued to increase with time after spraying 0.2 mmol·L- -1 SAC， which significantly increased by 44.57% and 83.22% after 12h compared with the control（CK）treatment. The results of fluorescence labeling on the leaves and roots of rice seedlings also showed that the fluorescence intensity of H2S significantly increased by 254.55% and 700.25% compared with CK treatment. The results of L-cysteine desulfurase（LCD）activity showed that the LCD activities in shoot and root of 0.2mmol⋅L-1 SAC were significantly increased by 94.17% and 41.05% compared with CK treatment， respectively. After spraying 0.2 mmol·L-1 SAC， the activities of superoxide dismutase（SOD）， peroxidase（POD）and catalase（CAT）in the shoot of rice seedlings were significantly increased by 32.36% ， 26.69% ， 24.87% ， respectively， the activity in the root was significantly increased by 38.61% ， 11.99% and 62.93% ， respectively， compared with CK treatment. The content of malondialdehyde（MDA）in shoot and root was significantly decreased by 37.37% and 28.54% ， respectively. The results showed that when the SAC spraying concentration reached 0.2mmol⋅L-1 ， the Cd contents in shoot and root of rice seedlings were significantly reduced by 18.07% and 46.05% ， respectively， compared with CK treatment. In conclusion， SAC spraying triggers the synthesis of H2S signaling molecules and regulates the alleviation of Cd stress in rice.

Keywords：rice; cadmium; S-allyl-L-cysteine; hydrogen sulfide; signaling molecule

土壤重金属污染已成为制约我国粮食安全生产的重要环境因素，其中又以土壤Cd 污染问题尤为突出。（剩余18648字）