2,3，7，8-四氯二苯并二嘌英通过miR-214-3p抑制间充质细胞的增殖和迁移导致聘裂的形成

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【中图分类号】R622.9；R726.2 【文献标志码】A

【Abstract】Objective：To investigate the role of miRNA（miR）-214-3p in a cleft palate induced by 2，3，7，8 -tetrachlorodibenzo-pdioxin（TCDD）.Methods： We establisheda cleft palate model by intragastrically administering TCDD （ 64|μg/kg ）to pregnant C57 mice.The expressionof miR-214-3p in mouse embryonic palatal mesenchymal（MEPM）celswas measured by miRNA sequencing andquantitativereal-timepolymerasechaireaction（qRT-PCR）.WeexamiedtheefectsofTCDDviamiR-214-3pontheprolferation and migration of MEPM cells by using Cell Counting Kit- 8，5- ethynyl- 2 '-deoxyuridine assay，transwell assay，and wound healing assay.Results：Exposing C57 mice at 10.5 days of gestation to TCDD（64 μg/kg），32 of 32 fetuses（ 100% ） in the experimental group developedacleftpalate.Nocleftpalateoccurredinthecontrolgroup（O/28）.TheTCDDgroupshowedsignificantlyup-regulatedexpressionofmiR-214-3p，miR-296-5p，andmiR-3-5pandsignificantlydown-regulatedexpresionofmiR-92a-3p，miR-126a-3p， and miR-411-5p.TheqRT-PCRresultsverifedmiR-214-3passignificantlydiferentiallyexpressed.ThemiR-214-3pihibitor partiallyrestoredteproliferationandmigrationabilitiesofEPMcellexpoedtoTCD.Conclusions：TCDDcansuppresthepro liferationand migrationof MEPMcelsthrough miR-214-3p，indicating thatmiR-214-3pmayplayacrucialrole in TCDD-induced cleft palate in mice.

【Keywords】miRNA-214-3p;mesenchymal cell;cleft palate;2，3，7，8-tetrachlorodibenzo- p -dioxin

腭裂（cleftpalate，CP）是全球第二大最常见的先天性残疾，大约每700个活产婴儿中就有1人患有此病。（剩余10764字）