FERMT1通过Wnt/β-catenin信号通路调控EMT促进肺腺癌转移的机制研究

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【中图分类号】R734.2

【文献标志码】A

【Abstract]Objective：To investigate theexpressionofFERMT1inlungadenocarcinomacellsandtheeffectand mechanismof FERMT1ontheproliferatio，vasionigration，ndapoptosisoflungadenocarioacelsbygulatingtheWntsigalingpathay. Methods：ThebioinformaticsanalysiswasusedtoinvestigatetheexpressonofFERM1inlungadenocarcinomaanditsassociation withclinicalfeaturesndprogosis;initroexperimentsreuedtoivestigateteifuenceofFERontiologicalavior of lung adenocarcioma;qRT-PCRandWesteblotwereusedtoobserve theefectofthechangeiFERMT1expresiononthe Wnt/β -cateninpathwayanditsdownstreamtargetgenes.Results：ThebioinformaticsanalysisshowedthehighexpresionofFERinlng adenocarcinomatissue，theexpressonlevelofFERMTinTNMstagingoflungadenocarcinomawashigherthanthatinnomalung tisue，andmeanhile，theighexpresioofFERledtotheoorprognosisoflungadenocarciomapatients，ainlythoughthe Wntsignalingpathway.InvitroxperimentsshowedthatibiionofFERMexpresioneducedtheproliferaton，migrationd invasionabilitiesoflungadenocarcinomacellsandincreasedtheapoptosisoflungadenocarcinomacells.TheresultsofqRTPCRand Western blot showed that the change in FERMT1 expression affected the expression of β- catenin，epithelial-mesenchymal transition-related markers，and their downstream target genes. Conclusion：FERMT1iscloselyassociated with the development andprogressionoflungadenocarcinoma through theWnt/β-catenin signaling pathway.

【KeyWords】FERMT1;lung adenocarcinoma;bioinformatics;Wnt/ β-catenin

肺癌是发病率和死亡率较高的原发性癌症类型，由于缺乏临床表现，大多数早期非小细胞肺癌患者错过了最佳治疗时机。（剩余9418字）