聚苯乙烯微塑料联合高脂处理对血管内皮细胞的影响

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【中图分类号】R114 【文献标志码】A

【收稿日期】2024-09-03

Effect of polystyrene microplastics combined with high-fat treatment onvascularendothelial cells

Wang Jing，Diao Jiani，Long Jing，He Yuguang，Tan Lipin，Chen Xuemei，Li Fangfang，He Junlin， WangYingxiong，GaoRufei，LiWeike （1Joint Laboratory ofInternational Cooperation ofthe Ministryof Education for Reproduction and Development， School ofPublic Health，Chongqing Medical University）

【AbstractObjective：ToinvestigatetheefectofpolystyrenemicroplasticsP-MPs）combinedwithhigh-fattreatmentonvascular endothelialcels.Methods ：Humanumbilicalveinendothelialcells（HUVECs）werecultured intheDMEMmediumcontaining 5% fetalbovineserum.HUVCsweretreatedwithconventionalculture，high-fattreatment，andPS-MPscombinedwithhigh-fattreatent. The experiment was conducted in the three groups of control group ，high-fat treatment group and PS-MPs + high-fat treatment group. CCK-8ssayasusedtomeasurecellviability-actinstaining wasuedtobseecellmorphologicalchanges，ndflcoetry， scratchassay，andtubeformationassywereusedtomeasuretheapoptosis，igrationandtub-formingabilityofcelsResults： AfterHUVECswerexpedtoeigtenviont，eresaicanductioinelvabilitysinagofelsifcanticreaseincellapoptosisndsigifiantrductonsincellgrationandtubeformingabilityomparedwithtegateat mentgroup，eatgsicelliltellolellopoisdellgratiilit combinedwithhigh-fattreatment，butthetube-formingabitofcellswasfurtherimpaired.Conclusion：High-fattreamentwill affectcellabilitangellorpolondmagslardotelialelluctiondsbidithat ment can aggravate the damage of vascular endothelial cell function.

【Key wordsIpolystyrene microplastics;high-fat;human umbilical veinendothelialcells;tube-forming ability

随着生活水平的提高和饮食结构的改变，肥胖人口数量飞速增长，肥胖的患病率也迅速增加。（剩余18752字）