凋亡外囊泡传递LncRNA-XIST在胶质瘤细胞耐药性作用机制研究

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【中图分类号】R739.41【收稿日期】2025-03-19

【AbstractObective：Toinvestigate theefectof glomacell-erivedapoptoticextracellarvesicles（apoEVs）onglomatumorigenesisand temozolomide（TMZ）resistanceandits mechanism.Methods：The extractedapoEVswerecharacterized using nanoparticle trackinganalsoodtelotfossolo Transwellsseedeatetelltigaaisu promotedTMZresistanceingliomacels，significantlyincreasedthehalf-maximalinhibitoryconcentrationof TMZ（t=9.326， P= （204号 0.001），andinbitedcellapoptois，andtiseccouldbeeversedbytheexosomeiibitorGW4869.ApoEValsoprootedthe migrationand invasion of glioma cells，increased the expression of vimentin and Twist proteins（t=8.762， P =0.002; t： =7.941，P=0.004）， and inhibited the expression of cleaved caspase -3 （t=9.217，P=O.oO2）.Further studies showed that apoEVs affected TMZ resistance of gliomacellsbyregulating theLncRNA-XIST/miR-29c/O6-methylguanine-DNAmethyltransferase（MGMT）axis.Silencingof LncRNA-XISTcould reduce theexpressonof MGMTandincrease theexpressionofmiR-29c，thereby enhancing thesensitivityto TMZandinhibitingcellmigrationandinvasion.Conclusion：Glomacell-erivedapEVpromote thealigantprogressionandteo zolomide resistance of glioma by delivering LncRNA-XIST to regulate the miR-29c/MGMT axis.

【Keywordslglioma;apoptoticextracellularvesicles;longon-coding RNAX-inactivespecific transcript;epithelial-mesenchmal transition;miR-29c/O6-methylguanine-DNA methyltransferase axis

神经胶质瘤是颅内最常见原发性恶性肿瘤，标准治疗包括最大限度的安全手术切除、术后放化疗联合电场治疗[]。（剩余13394字）