树突状细胞膜微泡在T细胞激活与结直肠癌细胞杀伤中的作用研究

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【中图分类号】R730.51 【文献标志码】A

Role of dendritic cell membrane microbubbles in the activation of T cells and thekilling of colorectal cancer cells

XuHan'，ZhangLiang²，LengXiaojing³，YanShujin'，PangHua'

（1.Departmentof NuclearMedicine，TheFirstAfliated Hospitalof Chongqing Medical University;2.Departmentof

Ultrasound，TheFirstAffiliated HospitalofChongqing Medical University;3.Departmentof Ultrasound Medicine， The Second Afliated Hospital of Chongqing Medical University）

【Abstract]Objective：Todesignandevaluateacellmembrane vaccinestrategy basedondendriticcell membrane microbubles （DCM@MBs）ndtexloreitsotentialplicatoninumorimunoterapyseiallyheimunespecific killngofrlls throughtheativationofTcells.Methods：Atfirst，tumorcellembraneproteiswereextractedanddendriccels（DCs）weractivatedtoconfirmthattumorantigenscouldeffectivelystimulatethematurationofimmatureDCs.MatureDC membraneswere then mixedwith lipidstoprepareCM@MBs，whichwereharacterizedformorphology，size，andproteincompositionbycofocallaser scaningmicrosopyandsumdodcylsulfate-polycrylamidegelelectrophoresis.Finallyinitrocoultureexperimentseoducted toassesstheeffectofCM@sontheactivationofTcellsandtheirabilitforspecifickillngoftumorcells.Results：Inthe in vitro DC activation experiment，after stimulation with tumor cell membrane proteins，the 25 μg/mL group had a significant increase in the expression level of MHC class II molecule （25.167%±1.203% ）on the surface of immature DCs compared with the control group（ P<0.001⟩ ，and DCM@MBs presented with microbubbles encapsulated byred cell membranes，with uniform dispersion and a sizeof 1-5μm .Inthein vitroco-cultureexperiment，theamount of INF-γ secreted byT cellswas 120.574±5.179 ） pg/mL in the DCM @ MBs group，significantly enhancing the activation of CD8+ T cells compared with the control group（ P <0.001），and furthermore， theimmune-specific killing rateagainst CT26.WT tumor cellswas （30.737±0.216）% ，which was 3.11 times that against 4T1 mouse breast cancer cells （9.893±0.341）% .Conclusion： The DCM@MBs strategy proposed in this study shows significant potential in tumorimmunotherayndcaneffeivelyctivateTcellsandspifcallykillandeliminatetumorcells，hchprovidesnwideasfortu mor immunotherapy.

【Keywords】dendritic cell;immunotherapy;microbubbles;cell membrane;dendritic cell vaccine

近年来，肿瘤免疫治疗已成为继传统手术、化疗和放疗之后的一种革命性治疗[1-2]。（剩余14564字）