一株鸭瘟病毒的分离鉴定和基因组特征

打开文本图片集

中图分类号：S852.659.1 文献标志码：A 文章编号：0366-6964（2026）02-1171-06

Isolation，Identification and Genomic Characterizationof a Duck Plague Virus

SONG Xiaofei1，LI Zhen1，PENG Jianyun1，JIA Aiqin1，WANG Miaoli，LI Fakai 2 ，LILei¹， ZHAO Yiqing1， QIN Xuwei¹， ZHAO Ni'， LI Fujin¹， ZHANG Qingchan ， LIU Huachun1， ZHANG Lianxiul* （1. Qilu Animal Health Products Co. Ltd.， Jinan 25o1Oo， China; 2.Shandong Provincial Animal Disease Prevention and Control Center Shandong Prouincial Animal Disease Prevention and Control Center [Shandong Provincial Zoonotic Disease Surveillance Center]， Jinan 25OlOo，China）

Abstract：From 2O23 to 2O24，1O-2O-day-old ducks in a meat duck farm in Jiangsu Province，were suspected of being infected with duck plague virus （DPV）.This study aims to isolate and identify the virus and analyze genomic characteristics. Viral isolation and genome sequencing analysis were performed on the disease material in this study. Results were as follows：Duck plague virus was detected in visceral tissue，and a duck plague strain was successfully isolated，named QL23l2. It has the same pathogenicity as the virulent strain. Sequence analysis of the isolate genome revealed that the UL2 gene of QL23l2 strain was the same as that of the vaccine strain C-KCE strain，while the UL56/ LORF5 gene was the same as the classical strain CHv and CV strains. This study successfully isolated a duck plague mutant strain，providing a basis for further research on related diseases.

Keywords： duck plague virus; separation and identification;genome analysis * Corresponding author： ZHANG Lianxiu，E-mail： lianxiu. zhang@qilu-pharma.com

鸭瘟病毒（duckplaque virus，DPV）是引起鸭、鹅和天鹅等禽类急性发病、败血性和高度致死性的一种传染病病原[1]。（剩余6992字）