IncRNA663调控CLDN10表达及猪流行性腹泻病毒复制的功能分析

打开文本图片集

中图分类号：Q522；S852.659.6 文献标志码：A 文章编号：0366-6964（2026）02-0945-10

Functional Analysis of IncRNA663 Regulating CLDN10 Expression and Porcine EpidemicDiarrheaVirusReplication

WU Zuolin，LIANG Haijiang，WU Zheyu， YANG Songbai， ZHAO Ayong，QIN Weiyun* （College of Animal Science and Technology·Colege of Veterinary Medicine， Zhejiang A&F University， Hangzhou 311300，China）

Abstract： Porcine epidemic diarrhea virus （PEDV） is a coronavirus that causes acute diarrhea， vomiting，and dehydration in piglets，among other clinical signs，and has caused severe economic losses to the global swine farming industry. More and more studies have reported that long noncoding RNAs （lncRNAs） play important regulatory roles in porcine coronaviruses，however，the role and mechanism of lncRNA 663 in PEDV infection remain unclear. Based on prior transcriptome sequencing，we identified a co-expression pattern between lncRNA 663 and the tight junction protein CLDN1O. This study aimed to explore their roles and regulatory relationship during PEDV infection. The qRT-PCR and Western blot results showed that the expresson levels of lncRNA 663 and

CLDN1O were up-regulated after PEDV infection of IPEC-J2 cells （ P<0.05） ），DSS treatment significantly reduced the expression levels of lncRNA 663 and CLDN10（ .P<0.05 ）.Subcellular localization prediction revealed that lncRNA 663 was mainly localized in the cytoplasm，and RPISeq prediction revealed that lncRNA663 and CLDN1O were highly interoperable （SVM： 0.98，RF ： 0.8）.Functional experiments demonstrated that siRNA-mediated knockdown of IncRNA 663 led to a significant decrease in both CLDNlO expression and PEDV replication （ P<0.05 ）.Furthermore， structure prediction using AlphaFold3 and ChimeraX revealed potential hydrogen bond interactions between CLDNlO and the PEDV spike （S） protein，suggesting a role in viral entry or infection. Collectively，these results suggest that IncRNA 663 may promote PEDV replication by regulating CLDN1O expression. These findings expand our understanding of PEDV-host interactions and provide a theoretical basis for future antiviral strategies targeting host non-coding RNAs.

Keywords： porcine epidemic diarrhea virus; long chain non-coding RNA; CLDN1O; intestinal barrier ∗ Corresponding author： QIN Weiyun，E-mail： qinwy@zafu. edu. cn

猪流行性腹泻（porcine epidemicdiarrhea，PED）是由猪流行性腹泻病毒（porcine epidemicdiarrheavirus，PEDV）引起的一种急性、高度接触性传染的肠道疾病。（剩余15671字）