白藜芦醇缓解热应激诱导猪丸支持细胞氧化应激的作用

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中图分类号：S828.3 文献标志码：A 文章编号：0366-6964（2026）02-0780-09

TheEffectofResveratrol on AlleviatingHeat Stress Induced Oxidative Stress in Porcine Sertoli Cells

ZHANG Li， RAN Maoliang* （Collge of Animal Science and Technology， Hunan Agricultural University，Changsha 410l28，China）

Abstract：This study aimed to investigate the impact of heat stress on the antioxidant capacity of porcine sertoli cells and to further elucidate the protective role and underlying mechanism of resveratrol （RES） in alleviating oxidative stress. Porcine sertoli cels were isolated and cultured primarily. The cells were divided into 3 groups： control group （NC， 34∘C ），heat stress group （HS， 43∘C for 30min ），and heat stress+resveratrol group （HS+200nmol⋅L-1RES） ）．The levels of reactive oxygen species （ROS），the content of malondialdehyde （MDA） and advanced glycation end products （AGEs），as well as the activities of superoxide dismutase（SOD） and catalase（CAT） were measured. The expression levels of RAGE ，NOX4，and NF-κB genes were determined using realtime fluorescence quantitative PCR. Compared with the control group，heat stress significantly increased ROS level （ ⋅P<0.05 ），MDAcontent（ P<0.05 ），and AGEs content（ P<0.01 ），whereas the activities of SOD and CAT were significantly decreased P<0.01i ），indicating that heat stress successfully induced oxidative stress in porcine sertoli cells. Pretreatment with 2OO nmol·L-1 RES significantly attenuated the heat stress-induced elevations in ROS，MDA，and AGEs levels （ P< 0.05），and restored the activities of SOD and CAT（ P<0.05 ）．Moreover，RES significantly downregulated the expression levels of RAGE ，NOX4，and NF-κB genes induced by heat stress （ P<0.05 ）.Heat stress disrupts redox homeostasis in porcine sertoli cells，leading to oxidative stress.Resveratrol alleviates this heat stress-induced oxidative stress by suppressing the AGEsRAGE/NOX4/NF- κB signaling pathway. The findings provide a theoretical basis for utilizing resveratrol to improve boar semen quality during summer.

Keywords：heat stress;porcine sertoli cells;resveratrol;oxidative stress ∗ Corresponding author： RAN Maoliang，E-mail： ranmaoliangO9O3@l26.com

环境高温是影响畜牧生产的重要不利因素之一，对畜禽机体健康和生殖机能均有重要影响[1-2]。（剩余16347字）